Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus

Eduardo L.V. Silveira, Kenneth A. Rogers, Sanjeev Gumber, Praveen Amancha, Peng Xiao, Shawna M. Woollard, Siddappa Nagadenahalli Byrareddy, Mauro Martins Teixeira, Francois Villinger

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Zika virus (ZIKV) is a mosquito-borne and sexually transmitted flavivirus that is associated with fetal CNS-damaging malformations during pregnancy in humans. This study documents the viral kinetics and immune responses in rhesus macaques infected with a clinical ZIKV Brazilian isolate. We evaluated the viral kinetics and immune responses induced after an i.v. infection with a Brazilian ZIKV clinical isolate (HS-2015-BA-01) in rhesus macaques for up to 142 d. ZIKV-specific Ab-secreting cells, germinal center reactions, and monocyte, dendritic cell, NK, and T cell frequencies were monitored. ZIKV loads were readily detected in plasma (until day 5 or 7), semen and urine (until days 7 and 14), and saliva (until day 42), but the viremia was rapidly controlled. No detectable clinical manifestations were observed. However, lymph node hyperplasia was clearly visible postviremia but was associated with low frequencies of ZIKV-specific Ab-secreting cells in lymph nodes and bone marrow, correlating with low Ab titers. CD14+/CD16- monocytes and myeloid CD11chi dendritic cells decreased in blood, whereas NK and T cell numbers were only marginally altered during the course of the study. ZIKV infection caused a significant lymphoid tissue activation but limited induction of ZIKV-specific B cells, suggesting that these parameters need to be considered for ZIKV vaccine design.

Original languageEnglish (US)
Pages (from-to)1003-1011
Number of pages9
JournalJournal of Immunology
Volume199
Issue number3
DOIs
StatePublished - Aug 1 2017

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Macaca mulatta
Natural Killer Cells
Dendritic Cells
Monocytes
Lymph Nodes
T-Lymphocytes
Flavivirus
Germinal Center
Zika Virus
Viremia
Lymphoid Tissue
Semen
Culicidae
Saliva
Hyperplasia
B-Lymphocytes
Vaccines
Cell Count
Bone Marrow
Urine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Silveira, E. L. V., Rogers, K. A., Gumber, S., Amancha, P., Xiao, P., Woollard, S. M., ... Villinger, F. (2017). Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus. Journal of Immunology, 199(3), 1003-1011. https://doi.org/10.4049/jimmunol.1700256

Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus. / Silveira, Eduardo L.V.; Rogers, Kenneth A.; Gumber, Sanjeev; Amancha, Praveen; Xiao, Peng; Woollard, Shawna M.; Byrareddy, Siddappa Nagadenahalli; Teixeira, Mauro Martins; Villinger, Francois.

In: Journal of Immunology, Vol. 199, No. 3, 01.08.2017, p. 1003-1011.

Research output: Contribution to journalArticle

Silveira, ELV, Rogers, KA, Gumber, S, Amancha, P, Xiao, P, Woollard, SM, Byrareddy, SN, Teixeira, MM & Villinger, F 2017, 'Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus', Journal of Immunology, vol. 199, no. 3, pp. 1003-1011. https://doi.org/10.4049/jimmunol.1700256
Silveira ELV, Rogers KA, Gumber S, Amancha P, Xiao P, Woollard SM et al. Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus. Journal of Immunology. 2017 Aug 1;199(3):1003-1011. https://doi.org/10.4049/jimmunol.1700256
Silveira, Eduardo L.V. ; Rogers, Kenneth A. ; Gumber, Sanjeev ; Amancha, Praveen ; Xiao, Peng ; Woollard, Shawna M. ; Byrareddy, Siddappa Nagadenahalli ; Teixeira, Mauro Martins ; Villinger, Francois. / Immune cell dynamics in rhesus macaques infected with a Brazilian strain of Zika virus. In: Journal of Immunology. 2017 ; Vol. 199, No. 3. pp. 1003-1011.
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