Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds

Bin Duan, Min Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.

Original languageEnglish (US)
Title of host publicationAdvances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8
Pages43-51
Number of pages9
StatePublished - Sep 1 2010
Event8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8 - Vancouver, BC, Canada
Duration: May 31 2009Jun 5 2009

Publication series

NameCeramic Transactions
Volume218
ISSN (Print)1042-1122

Other

Other8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8
CountryCanada
CityVancouver, BC
Period5/31/096/5/09

Fingerprint

Gelatin
Scaffolds (biology)
Scaffolds
Heparin
Nanocomposites
Intercellular Signaling Peptides and Proteins
Struts
Surface treatment
Bone
Tolonium Chloride
Tissue regeneration
Hydrophilicity
Cell proliferation
Polysaccharides
Hydrophobicity
Tissue engineering
Vascular Endothelial Growth Factor A
Assays
Sintering
Degradation

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

Cite this

Duan, B., & Wang, M. (2010). Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds. In Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8 (pp. 43-51). (Ceramic Transactions; Vol. 218).

Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds. / Duan, Bin; Wang, Min.

Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8. 2010. p. 43-51 (Ceramic Transactions; Vol. 218).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Duan, B & Wang, M 2010, Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds. in Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8. Ceramic Transactions, vol. 218, pp. 43-51, 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8, Vancouver, BC, Canada, 5/31/09.
Duan B, Wang M. Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds. In Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8. 2010. p. 43-51. (Ceramic Transactions).
Duan, Bin ; Wang, Min. / Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds. Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8. 2010. pp. 43-51 (Ceramic Transactions).
@inproceedings{4b7395de876f44918ed25605c7ad8634,
title = "Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds",
abstract = "In bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.",
author = "Bin Duan and Min Wang",
year = "2010",
month = "9",
day = "1",
language = "English (US)",
isbn = "9780470905487",
series = "Ceramic Transactions",
pages = "43--51",
booktitle = "Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8",

}

TY - GEN

T1 - Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds

AU - Duan, Bin

AU - Wang, Min

PY - 2010/9/1

Y1 - 2010/9/1

N2 - In bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.

AB - In bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=77956026942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956026942&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:77956026942

SN - 9780470905487

T3 - Ceramic Transactions

SP - 43

EP - 51

BT - Advances in Bioceramics and Biotechnologies - A Collection of Papers Presented at the 8th Pacific Rim Conference on Ceramic and Glass Technology, PACRIM-8

ER -