Immobilization of α1-acid glycoprotein for chromatographic studies of drug-protein binding II. correction for errors in association constant measurements

Rangan Mallik, Hai Xuan, Georges Guiochon, David S. Hage

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A new method for the immobilization of α1-acid glycoprotein (AGP) in high-performance liquid chromatography (HPLC) columns was recently described for applications such as drug binding studies. Part of this earlier work used self-competition zonal elution studies to measure association equilibrium constants between immobilized AGP and R- or S-propranolol. It was later found that analysis of these data by a common equation derived for linear elution conditions gave erroneous values for experiments actually conducted under nonlinear conditions. This report discusses the nature of this error and uses frontal analysis to estimate the true binding strength between R- and S-propranolol and HPLC columns containing immobilized AGP.

Original languageEnglish (US)
Pages (from-to)154-156
Number of pages3
JournalAnalytical Biochemistry
Volume376
Issue number1
DOIs
StatePublished - May 1 2008

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Protein Binding
Immobilization
Glycoproteins
Association reactions
High performance liquid chromatography
Propranolol
Acids
High Pressure Liquid Chromatography
Pharmaceutical Preparations
Equilibrium constants
Experiments

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Immobilization of α1-acid glycoprotein for chromatographic studies of drug-protein binding II. correction for errors in association constant measurements. / Mallik, Rangan; Xuan, Hai; Guiochon, Georges; Hage, David S.

In: Analytical Biochemistry, Vol. 376, No. 1, 01.05.2008, p. 154-156.

Research output: Contribution to journalArticle

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