Imaging DNA molecules chemically bound to a mica surface (Invited Paper)

Stuart M. Lindsay, Y. L. Lyubchenko, A. A. Gall, L. S. Shlyakhtenko, R. E. Harrington

Research output: Chapter in Book/Report/Conference proceedingConference contribution

12 Citations (Scopus)

Abstract

We describe a procedure for imaging DNA molecules using atomic force microscopy (AFM). Double-stranded DNA binds to a mica surface modified by treatment with 3- aminopropyltriethoxysilane to yield AFM images that are stable under repeated scanning. We used this procedure to obtain high quality images of unstained linear DNA molecules and circular DNA molecules. The resolution was limited to tens of nanometers by the profile of the AFM tips.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages84-90
Number of pages7
ISBN (Print)0819407852
StatePublished - Jan 1 1992
EventScanning Probe Microscopies - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 22 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1639
ISSN (Print)0277-786X

Other

OtherScanning Probe Microscopies
CityLos Angeles, CA, USA
Period1/20/921/22/92

Fingerprint

Mica
mica
DNA
deoxyribonucleic acid
Imaging techniques
Atomic force microscopy
Molecules
atomic force microscopy
molecules
Image quality
Scanning
scanning
profiles

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Lindsay, S. M., Lyubchenko, Y. L., Gall, A. A., Shlyakhtenko, L. S., & Harrington, R. E. (1992). Imaging DNA molecules chemically bound to a mica surface (Invited Paper). In Proceedings of SPIE - The International Society for Optical Engineering (pp. 84-90). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1639). Publ by Int Soc for Optical Engineering.

Imaging DNA molecules chemically bound to a mica surface (Invited Paper). / Lindsay, Stuart M.; Lyubchenko, Y. L.; Gall, A. A.; Shlyakhtenko, L. S.; Harrington, R. E.

Proceedings of SPIE - The International Society for Optical Engineering. Publ by Int Soc for Optical Engineering, 1992. p. 84-90 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1639).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lindsay, SM, Lyubchenko, YL, Gall, AA, Shlyakhtenko, LS & Harrington, RE 1992, Imaging DNA molecules chemically bound to a mica surface (Invited Paper). in Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 1639, Publ by Int Soc for Optical Engineering, pp. 84-90, Scanning Probe Microscopies, Los Angeles, CA, USA, 1/20/92.
Lindsay SM, Lyubchenko YL, Gall AA, Shlyakhtenko LS, Harrington RE. Imaging DNA molecules chemically bound to a mica surface (Invited Paper). In Proceedings of SPIE - The International Society for Optical Engineering. Publ by Int Soc for Optical Engineering. 1992. p. 84-90. (Proceedings of SPIE - The International Society for Optical Engineering).
Lindsay, Stuart M. ; Lyubchenko, Y. L. ; Gall, A. A. ; Shlyakhtenko, L. S. ; Harrington, R. E. / Imaging DNA molecules chemically bound to a mica surface (Invited Paper). Proceedings of SPIE - The International Society for Optical Engineering. Publ by Int Soc for Optical Engineering, 1992. pp. 84-90 (Proceedings of SPIE - The International Society for Optical Engineering).
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