IL13 activates autophagy to regulate secretion in airway epithelial cells

John D. Dickinson, Yael Alevy, Nicole P. Malvin, Khushbu K. Patel, Sean P. Gunsten, Michael J. Holtzman, Thaddeus S. Stappenbeck, Steven L. Brody

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cytokine modulation of autophagy is increasingly recognized in disease pathogenesis, and current concepts suggest that type 1 cytokines activate autophagy, whereas type 2 cytokines are inhibitory. However, this paradigm derives primarily from studies of immune cells and is poorly characterized in tissue cells, including sentinel epithelial cells that regulate the immune response. In particular, the type 2 cytokine IL13 (interleukin 13) drives the formation of airway goblet cells that secrete excess mucus as a characteristic feature of airway disease, but whether this process is influenced by autophagy was undefined. Here we use a mouse model of airway disease in which IL33 (interleukin 33) stimulation leads to IL13-dependent formation of airway goblet cells as tracked by levels of mucin MUC5AC (mucin 5AC, oligomeric mucus/gel forming), and we show that these cells manifest a block in mucus secretion in autophagy gene Atg16l1-deficient mice compared to wild-type control mice. Similarly, primary-culture human tracheal epithelial cells treated with IL13 to stimulate mucus formation also exhibit a block in MUC5AC secretion in cells depleted of autophagy gene ATG5 (autophagy-related 5) or ATG14 (autophagyrelated 14) compared to nondepleted control cells. Our findings indicate that autophagy is essential for airway mucus secretion in a type 2, IL13-dependent immune disease process and thereby provide a novel therapeutic strategy for attenuating airway obstruction in hypersecretory inflammatory diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis lung disease. Taken together, these observations suggest that the regulation of autophagy by Th2 cytokines is cell-context dependent.

Original languageEnglish (US)
Pages (from-to)397-409
Number of pages13
JournalAutophagy
Volume12
Issue number2
DOIs
StatePublished - Jan 1 2016

Fingerprint

Interleukin-13
Autophagy
Epithelial Cells
Mucus
Cytokines
Goblet Cells
Mucin 5AC
Immune System Diseases
Mucins
Airway Obstruction
Cystic Fibrosis
Chronic Obstructive Pulmonary Disease
Lung Diseases
Genes
Asthma
Gels

Keywords

  • Airway epithelial cells
  • Autophagy
  • IL13
  • MUC5AC
  • Reactive oxygen species
  • Secretion

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Dickinson, J. D., Alevy, Y., Malvin, N. P., Patel, K. K., Gunsten, S. P., Holtzman, M. J., ... Brody, S. L. (2016). IL13 activates autophagy to regulate secretion in airway epithelial cells. Autophagy, 12(2), 397-409. https://doi.org/10.1080/15548627.2015.1056967

IL13 activates autophagy to regulate secretion in airway epithelial cells. / Dickinson, John D.; Alevy, Yael; Malvin, Nicole P.; Patel, Khushbu K.; Gunsten, Sean P.; Holtzman, Michael J.; Stappenbeck, Thaddeus S.; Brody, Steven L.

In: Autophagy, Vol. 12, No. 2, 01.01.2016, p. 397-409.

Research output: Contribution to journalArticle

Dickinson, JD, Alevy, Y, Malvin, NP, Patel, KK, Gunsten, SP, Holtzman, MJ, Stappenbeck, TS & Brody, SL 2016, 'IL13 activates autophagy to regulate secretion in airway epithelial cells', Autophagy, vol. 12, no. 2, pp. 397-409. https://doi.org/10.1080/15548627.2015.1056967
Dickinson JD, Alevy Y, Malvin NP, Patel KK, Gunsten SP, Holtzman MJ et al. IL13 activates autophagy to regulate secretion in airway epithelial cells. Autophagy. 2016 Jan 1;12(2):397-409. https://doi.org/10.1080/15548627.2015.1056967
Dickinson, John D. ; Alevy, Yael ; Malvin, Nicole P. ; Patel, Khushbu K. ; Gunsten, Sean P. ; Holtzman, Michael J. ; Stappenbeck, Thaddeus S. ; Brody, Steven L. / IL13 activates autophagy to regulate secretion in airway epithelial cells. In: Autophagy. 2016 ; Vol. 12, No. 2. pp. 397-409.
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