IL-4 and IL-13 Induce Chemotaxis of Human Foreskin Fibroblasts, but Not Human Fetal Lung Fibroblasts

Tadashi Kohyama, Xiang-de Liu, Fu Qiang Wen, Tetsu Kobayashi, Shinji Abe, Stephen I. Rennard

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Through shared receptors, IL-4 and IL-13 have been suggested to regulate not only inflammatory cells, but also to play a role in stimulating fibroblasts during fibrotic processes. Previous studies have shown that IL-4 is a chemoattractant for foreskin fibroblasts. The current study was designed to determine the effect of IL-4 and IL-13 on the migration of two types of fibroblasts: foreskin and human fetal lung fibroblasts (HFL-1). Using the Boyden blindwell chamber method, human foreskin or fetal lung fibroblasts (both 10 6 /mL) were placed in upper wells with various concentrations of IL-4 or IL-13 in the lower wells as chemoattractants. Both IL-4 (1 pg/mL) and IL-13 (100 pg/mL) induced foreskin fibroblast chemotaxis, up to 50 ± 8 and 24 ± 7 cells/5 high-power fields, respectively (both p < 0.05). In contrast, neither cytokine induced migration of the lung fibroblasts although both type of cells express IL-4 receptor and IL-13 α1 receptor. These results suggest that fibroblasts are heterogeneous with regard to their ability to respond to cytokine-driven chemotaxis. Therefore, the role of specific cytokines in mediating fibrotic responses might vary depending on local mesenchymal cell responses.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalInflammation
Volume28
Issue number1
DOIs
StatePublished - Feb 1 2004

Fingerprint

Foreskin
Interleukin-13
Chemotaxis
Interleukin-4
Fibroblasts
Lung
Interleukin-4 Receptors
Chemotactic Factors
Cytokines
Interleukin-13 Receptors
Aptitude
Interleukin-1 Receptors
Interleukin-1

Keywords

  • Airway remodeling
  • Chemotaxis
  • Fibroblasts
  • IL-13
  • Interleukin-4 (IL-4)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

IL-4 and IL-13 Induce Chemotaxis of Human Foreskin Fibroblasts, but Not Human Fetal Lung Fibroblasts. / Kohyama, Tadashi; Liu, Xiang-de; Wen, Fu Qiang; Kobayashi, Tetsu; Abe, Shinji; Rennard, Stephen I.

In: Inflammation, Vol. 28, No. 1, 01.02.2004, p. 33-37.

Research output: Contribution to journalArticle

Kohyama, Tadashi ; Liu, Xiang-de ; Wen, Fu Qiang ; Kobayashi, Tetsu ; Abe, Shinji ; Rennard, Stephen I. / IL-4 and IL-13 Induce Chemotaxis of Human Foreskin Fibroblasts, but Not Human Fetal Lung Fibroblasts. In: Inflammation. 2004 ; Vol. 28, No. 1. pp. 33-37.
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