IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Yuannan Xia, D. E. Burbank, L. Uher, D. Rabussay, James L Van Etten

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

Original languageEnglish (US)
Pages (from-to)6075-6090
Number of pages16
JournalNucleic acids research
Volume15
Issue number15
DOIs
StatePublished - Jan 1 1987

Fingerprint

Chlorella
Chlorophyta
Deoxyribonuclease I
DNA Restriction Enzymes
Virus Diseases
Type II Site Specific Deoxyribonucleases
S-Adenosylmethionine
Dithiothreitol
Cytosine
DNA
Methylation
Adenosine Triphosphate
Viruses

ASJC Scopus subject areas

  • Genetics

Cite this

IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity. / Xia, Yuannan; Burbank, D. E.; Uher, L.; Rabussay, D.; Van Etten, James L.

In: Nucleic acids research, Vol. 15, No. 15, 01.01.1987, p. 6075-6090.

Research output: Contribution to journalArticle

@article{07021cf58aad4a719490c9f83c17cbd1,
title = "IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.",
abstract = "A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This {"}star{"} activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor {"}star{"} activity of other restriction endonucleases.",
author = "Yuannan Xia and Burbank, {D. E.} and L. Uher and D. Rabussay and {Van Etten}, {James L}",
year = "1987",
month = "1",
day = "1",
doi = "10.1093/nar/15.15.6075",
language = "English (US)",
volume = "15",
pages = "6075--6090",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "15",

}

TY - JOUR

T1 - IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

AU - Xia, Yuannan

AU - Burbank, D. E.

AU - Uher, L.

AU - Rabussay, D.

AU - Van Etten, James L

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

AB - A type II restriction endonuclease, named CviJI, was isolated from a eukaryotic Chlorella-like green alga infected with the dsDNA containing virus IL-3A. CviJI is the first restriction endonuclease to recognize the sequence PuGCPy; CviJI cleaves DNA between the G and C. Methylation of the cytosine in PuGCPy sequences prevents cleavage by CviJI. CviJI cleaved DNA into smaller but defined fragments in the presence of ATP. This "star" activity was stimulated by dithiothreitol and/or S-adenosylmethionine but did not occur under conditions which favor "star" activity of other restriction endonucleases.

UR - http://www.scopus.com/inward/record.url?scp=0023651157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023651157&partnerID=8YFLogxK

U2 - 10.1093/nar/15.15.6075

DO - 10.1093/nar/15.15.6075

M3 - Article

VL - 15

SP - 6075

EP - 6090

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 15

ER -