IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth.

María José Gravisaco, Claudia Mongini, Elida Alvarez, Paula Ruybal, Ana Escalada, Mariano Sanchez-Lockhart, Silvia Hajos, Claudia Waldner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We studied the role of IL-2, IL-15, IL-10, TNF and IL-2 receptor complexes (IL-2R) produced constitutively by a T-cell lymphoma line (LBC) on their own proliferation. The constitutive expression of surface alpha, beta and gamma chains IL-2R was detected in tumor cells by flow cytometry. Using reverse-transcription PCR, mRNA for IL-2, IL-15, IL-10 and TNF were found to be present in LBC. In addition, tumor cells were found to constitutively express intracellular IL-2, IL-15, IL-10 and TNF. Despite the production of these cytokines by tumor cells, specific neutralising antibodies did not inhibit LBC proliferation; surprisingly, anti-IL-15 increased LBC cell growth. We also demonstrated that recombinant IL-2 or IL-15 enhanced LBC cell proliferation. Our data suggest that endogenous IL-2 and IL-15 may trigger the proliferation of lymphoma LBC cells, and so their growth could be regulated, at least partly, by IL-2/IL-15/IL-2R system. In addition, IL-10 and TNF, immunosuppressor and pro-metastatic cytokines, respectively, may promote the in vivo growth of the tumor. The fact that leukaemia-lymphoma cells produce simultaneously both IL-2 and IL-15 should be taken into consideration in the design of immunotherapy protocols directed to IL-2R.

Original languageEnglish (US)
Pages (from-to)627-632
Number of pages6
JournalInternational journal of molecular medicine
Volume12
Issue number4
StatePublished - Jan 1 2003

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Interleukin-15
T-Cell Lymphoma
Interleukin-10
Interleukin-2
Interleukin-2 Receptors
Growth
Lymphoma
Neoplasms
Cytokines
Neutralizing Antibodies
Immunotherapy
Reverse Transcription
Flow Cytometry
Leukemia
Cell Proliferation
Cell Line
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Gravisaco, M. J., Mongini, C., Alvarez, E., Ruybal, P., Escalada, A., Sanchez-Lockhart, M., ... Waldner, C. (2003). IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth. International journal of molecular medicine, 12(4), 627-632.

IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth. / Gravisaco, María José; Mongini, Claudia; Alvarez, Elida; Ruybal, Paula; Escalada, Ana; Sanchez-Lockhart, Mariano; Hajos, Silvia; Waldner, Claudia.

In: International journal of molecular medicine, Vol. 12, No. 4, 01.01.2003, p. 627-632.

Research output: Contribution to journalArticle

Gravisaco, MJ, Mongini, C, Alvarez, E, Ruybal, P, Escalada, A, Sanchez-Lockhart, M, Hajos, S & Waldner, C 2003, 'IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth.', International journal of molecular medicine, vol. 12, no. 4, pp. 627-632.
Gravisaco, María José ; Mongini, Claudia ; Alvarez, Elida ; Ruybal, Paula ; Escalada, Ana ; Sanchez-Lockhart, Mariano ; Hajos, Silvia ; Waldner, Claudia. / IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth. In: International journal of molecular medicine. 2003 ; Vol. 12, No. 4. pp. 627-632.
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