IL-13 is transcriptionally expressed in IgG-immune complex-induced lung injury

Larry D. Crouch, Thomas P. Shanley, Kent J. Johnson, Peter A. Ward

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

IL-13 suppresses in vitro production of many proinflammatory factors. Initial in vivo experiments indicate that intratracheally administered IL-13 attenuates the increased vascular permeability that is observed in IgGimmune complex-mediated lung injury. To elucidate whether endogenous IL-13 is generated in the course of the inflammatory response in vivo, we sought to determine whether IL-13 was transcriptionally expressed during IgG-immune complex-induced lung injury. Rat IL-13 was PCR cloned from cDNA templates generated by the reverse transcription of mRNA isolated from injured rat lungs. Using a sequential PCR strategy that employed two sets of primers constructed according to the published rat IL-13 sequence, a PCR product was obtained and ligated into a pCR II vector, sequenced and confirmed to be rat IL-13. This clone was then utilized to generate a 32p probe for IL13 for use in Northern blot analysis of whole lung RNA obtained over the time course of IgG-immune complex-induced injury. The results demonstrated that IL-13 message increased 10% to 75% above baseline expression during the first 24 hours following injury. These findings suggest that IL-13 may function as an endogenous regulator of the in vivo inflammatory response during IgG-immune complex-induced lung njury.

Original languageEnglish (US)
Pages (from-to)A1008
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

antigen-antibody complex
Interleukin-13
Lung Injury
Antigen-Antibody Complex
Immunoglobulin G
lungs
Rats
rats
Polymerase Chain Reaction
Lung
inflammation
interleukin-13
reverse transcription
Wounds and Injuries
Capillary Permeability
blood vessels
Northern blotting
Transcription
Northern Blotting
permeability

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Crouch, L. D., Shanley, T. P., Johnson, K. J., & Ward, P. A. (1996). IL-13 is transcriptionally expressed in IgG-immune complex-induced lung injury. FASEB Journal, 10(6), A1008.

IL-13 is transcriptionally expressed in IgG-immune complex-induced lung injury. / Crouch, Larry D.; Shanley, Thomas P.; Johnson, Kent J.; Ward, Peter A.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996, p. A1008.

Research output: Contribution to journalArticle

Crouch, LD, Shanley, TP, Johnson, KJ & Ward, PA 1996, 'IL-13 is transcriptionally expressed in IgG-immune complex-induced lung injury', FASEB Journal, vol. 10, no. 6, pp. A1008.
Crouch, Larry D. ; Shanley, Thomas P. ; Johnson, Kent J. ; Ward, Peter A. / IL-13 is transcriptionally expressed in IgG-immune complex-induced lung injury. In: FASEB Journal. 1996 ; Vol. 10, No. 6. pp. A1008.
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