Identification of Two Distinct Pathways of Protein Kinase Cα Down-regulation in Intestinal Epithelial Cells

Olga V. Leontieva, Jennifer D. Black

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Signal transduction pathways are controlled by desensitization mechanisms, which can affect receptors and/or downstream signal transducers. It has long been recognized that members of the protein kinase C (PKC) family of signal transduction molecules undergo down-regulation in response to activation. Previous reports have indicated that key steps in PKCα desensitization include caveolar internalization, priming site dephosphorylation, ubiquitination of the dephosphorylated protein, and degradation by the proteasome. In the current study, comparative analysis of PKCα processing induced by the PKC agonists phorbol 12-myristate 13-acetate and bryostatin 1 in IEC-18 rat intestinal epithelial cells demonstrates that: (a) at least two pathways of PKCα down-regulation can co-exist within cells, and (b) a single PKC agonist can activate both pathways at the same time. Using a combined biochemical and morphological approach, we identify a novel pathway of PKCα desensitization that involves ubiquitination of mature, fully phosphorylated activated enzyme at the plasma membrane and subsequent down-regulation by the proteasome. The phosphatase inhibitors okadaic acid and calyculin A accelerated PKCα down-regulation and inhibitors of vesicular trafficking did not prevent degradation of the protein, indicating that neither internalization nor priming site dephosphorylation are requisite intermediate steps in this ubiquitin/proteasome dependent pathway of PKCα down-regulation. Instead, caveolar trafficking and dephosphorylation are involved in a second, proteasome-independent mechanism of PKCα desensitization in this system. Our findings highlight subcellular distribution and phosphorylation state as critical determinants of PKCα desensitization pathways.

Original languageEnglish (US)
Pages (from-to)5788-5801
Number of pages14
JournalJournal of Biological Chemistry
Volume279
Issue number7
DOIs
StatePublished - Feb 13 2004

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Protein Kinase C
Down-Regulation
Epithelial Cells
Proteasome Endopeptidase Complex
Signal transduction
Ubiquitination
Proteolysis
Signal Transduction
Degradation
Okadaic Acid
Phosphorylation
Corrosion inhibitors
Cell membranes
Ubiquitin
Transducers
Phosphoric Monoester Hydrolases
Rats
Proteins
Acetates
Chemical activation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Identification of Two Distinct Pathways of Protein Kinase Cα Down-regulation in Intestinal Epithelial Cells. / Leontieva, Olga V.; Black, Jennifer D.

In: Journal of Biological Chemistry, Vol. 279, No. 7, 13.02.2004, p. 5788-5801.

Research output: Contribution to journalArticle

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