Identification of the transactivation domain of the transcription factor Sox-2 and an associated co-activator

Tamara K. Nowling, Lance R. Johnson, Matthew S Wiebe, A Angie Rizzino

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The importance of interactions between Sox and POU transcription factors in the regulation of gene expression is becoming increasingly apparent. Recently, many examples of the involvement of Sox-POU partnerships in transcription have been discovered, including a partnership between Sox-2 and Oct-3. Little is known about the mechanisms by which these factors modulate transcription. To better understand the molecular interactions involved, we mapped the location of the transactivation domain of Sox-2. This was done in the context of its interaction with Oct-3, as well as its ability to transactivate as a fusion protein linked to the DNA-binding domain of Gal4. Both approaches demonstrated that Sox-2 contains a transactivation domain in its C-terminal half, containing a serine-rich region and the C terminus. We also determined that the viral oncoprotein E1a inhibits the ability of the Gal4/Sox-2 fusion protein to transactivate, as well as the transcriptional activation mediated by the combined action of Sox-2 and Oct-3. In contrast, a mutant form of E1a, unable to bind p300, lacks both of these effects. Importantly, we determined that p300 overcomes the inhibitory effects of E1a in both assays. Together, these findings suggest that Sox-2 mediates its effects, at least in part, through the co-activator p300.

Original languageEnglish (US)
Pages (from-to)3810-3818
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number6
DOIs
StatePublished - Feb 11 2000

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Transcriptional Activation
Transcription Factors
Fusion reactions
POU Domain Factors
Molecular interactions
Oncogene Proteins
Transcription
Gene expression
Serine
Assays
Proteins
Chemical activation
Gene Expression Regulation
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of the transactivation domain of the transcription factor Sox-2 and an associated co-activator. / Nowling, Tamara K.; Johnson, Lance R.; Wiebe, Matthew S; Rizzino, A Angie.

In: Journal of Biological Chemistry, Vol. 275, No. 6, 11.02.2000, p. 3810-3818.

Research output: Contribution to journalArticle

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