Identification of the gene encoding the alternative sigma factor σ(B) from Listera monocytogenes and its role in osmotolerance

Lynne A. Becker, Mehmet Sevket Çetin, Robert W Hutkins, Andrew K Benson

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Abstract

Listeria monocytogenes is well known for its robust physiology, which permits growth at low temperatures under conditions of high osmolarity and low pH. Although studies have provided insight into the mechanisms used by L. monocytogenes to allay the physiological consequences of these adverse environments, little is known about how these responses are coordinated. In the studies presented here, we have cloned the sigB gene and several rsb genes from L. monocytogenes, encoding homologs of the alternative sigma factor σ(B) anti the RsbUVWX proteins, which govern transcription of a general stress regulon in the related bacterium Bacillus subtilis. The L. monocytogenes and B. subtilis sigB and rsb genes are similar in sequence and physical organization; however, we observed that the activity of σ(B) in L. monocytogenes was uniquely responsive to osmotic upshifting, temperature downshifting, and the presence of EDTA in the growth medium. The magnitude of the response was greatest after an osmotic upshift, suggesting a role for σ(B) in coordinating osmotic responses in L. monocytogenes. A null mutation in the sigB gene led to substantial defects in the ability of L. monocytogenes to use betaine and carnitine as osmoprotectants. Subsequent measurements of betaine transport confirmed that the absence of σ(B) reduced the ability of the cells to accumulate betaine. Thus, σ(B) coordinates responses to a variety of physical and chemical signals, and its function facilitates the growth of L. monocytogenes under conditions of high osmotic strength.

Original languageEnglish (US)
Pages (from-to)4547-4554
Number of pages8
JournalJournal of bacteriology
Volume180
Issue number17
StatePublished - Sep 1 1998

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Sigma Factor
Listeria monocytogenes
Betaine
Genes
Bacillus subtilis
Growth
Regulon
Temperature
Carnitine
Edetic Acid
Osmolar Concentration
Bacteria
Mutation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Identification of the gene encoding the alternative sigma factor σ(B) from Listera monocytogenes and its role in osmotolerance. / Becker, Lynne A.; Çetin, Mehmet Sevket; Hutkins, Robert W; Benson, Andrew K.

In: Journal of bacteriology, Vol. 180, No. 17, 01.09.1998, p. 4547-4554.

Research output: Contribution to journalArticle

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