Identification of the ATPase subunit of the primary maltose transporter in the hyperthermophilic anaerobe Thermotoga maritima

Raghuveer Singh, Derrick White, Paul Blum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Thermotoga maritima is a hyperthermophilic anaerobic bacterium that produces molecular hydrogen (H2) by fermentation. It catabolizes a broad range of carbohydrates through the action of diverse ABC transporters. However, in T. maritima and related species, highly similar genes with ambiguous annotation obscure a precise understanding of genome function. In T. maritima, three putative malK genes, all annotated as ATPase subunits, exhibited high identity to each other. To distinguish between these genes, malK disruption mutants were constructed by gene replacement, and the resulting mutant cell lines were characterized. Only a disruption of malK3 produced a defect in maltose catabolism. To verify that the mutant phenotype arose specifically from malK3 inactivation, the malK3 mutation was repaired by recombination, and maltose catabolism was restored. This study demonstrates the importance of a maltose ABC-type transporter and its relationship to sugar metabolism in T. maritima.

Original languageEnglish (US)
Article numbere00930
JournalApplied and environmental microbiology
Volume83
Issue number18
DOIs
StatePublished - Sep 1 2017

Fingerprint

Thermotoga maritima
anaerobes
Maltose
maltose
adenosinetriphosphatase
Adenosine Triphosphatases
transporters
ABC transporters
ATP-Binding Cassette Transporters
catabolism
gene
mutants
Genes
metabolism
genes
Anaerobic Bacteria
gene targeting
Genetic Recombination
recombination
hydrogen

Keywords

  • ABC transporter
  • Genetic systems
  • Homologous recombination
  • Hydrogen
  • Hyperthermophile
  • Maltose

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Identification of the ATPase subunit of the primary maltose transporter in the hyperthermophilic anaerobe Thermotoga maritima. / Singh, Raghuveer; White, Derrick; Blum, Paul.

In: Applied and environmental microbiology, Vol. 83, No. 18, e00930, 01.09.2017.

Research output: Contribution to journalArticle

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