Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1

Thomas O Powers, A. L. Szalanski, P. G. Mullin, T. S. Harris, T. Bertozzi, J. A. Griesbach

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A molecular analysis of eight described species of seed gall nematode, along with six undescribed isolates from different hosts, has revealed a strong association between nucleotide sequence polymorphism and host status. Each anguinid nematode associated with a unique host produced a unique PCR-RFLP pattern for the ITS1 region. Anguina species that had been synonymized in the past, Anguina agrostis, A. funesta, and A. wevelli (Afrina wevwlli), were readily discriminated. Two undescribed species from northern New South Wales and southeastern South Australia, reported to be vectors of Rathyaibacter toxicus in the disease called "floodplain stagger," were differentiated by a single restriction enzyme, and both could be separated easily from A. funesta, the vector of R. toxicus in annual ryegrass toxicity. Other species differentiated in this study include A. agropyronifloris, A. graminis, A. microlaenae, A. pacificae, and undescrihed species from host species Dactylis glomerata, Agrostis avenacea, Polypogon monospeliensis, Stipa sp., Astrebla pectinata, and Holcus lanatus. Phylogenetic analysis of the ITS1 region suggests that considerable anguinid genetic diversification has accompanied specialization on different host species.

Original languageEnglish (US)
Pages (from-to)191-194
Number of pages4
JournalJournal of Nematology
Volume33
Issue number4
StatePublished - Dec 2001

Fingerprint

galls
restriction fragment length polymorphism
Nematoda
seeds
Agrostis avenacea
Astrebla
Polypogon
Anguina
Holcus lanatus
Stipa
Dactylis glomerata
Lolium
South Australia
New South Wales
floodplains
genetic polymorphism
toxicity
nucleotide sequences
phylogeny
enzymes

Keywords

  • Afrina wevwlli
  • Anguina agrostis
  • Anguina tritici
  • Diagnostics
  • Nematode identification
  • PCR-RFLP
  • Regulatory nematology
  • Seed gall nematode

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Powers, T. O., Szalanski, A. L., Mullin, P. G., Harris, T. S., Bertozzi, T., & Griesbach, J. A. (2001). Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology, 33(4), 191-194.

Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. / Powers, Thomas O; Szalanski, A. L.; Mullin, P. G.; Harris, T. S.; Bertozzi, T.; Griesbach, J. A.

In: Journal of Nematology, Vol. 33, No. 4, 12.2001, p. 191-194.

Research output: Contribution to journalArticle

Powers, TO, Szalanski, AL, Mullin, PG, Harris, TS, Bertozzi, T & Griesbach, JA 2001, 'Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1', Journal of Nematology, vol. 33, no. 4, pp. 191-194.
Powers TO, Szalanski AL, Mullin PG, Harris TS, Bertozzi T, Griesbach JA. Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. Journal of Nematology. 2001 Dec;33(4):191-194.
Powers, Thomas O ; Szalanski, A. L. ; Mullin, P. G. ; Harris, T. S. ; Bertozzi, T. ; Griesbach, J. A. / Identification of seed gall nematodes of agronomic and regulatory concern with PCR-RFLP of ITS1. In: Journal of Nematology. 2001 ; Vol. 33, No. 4. pp. 191-194.
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