Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast

Yashitola Jamir, Ming Guo, Hye Sook Oh, Tanja Petnicki-Ocwieja, Shaorong Chen, Xiaoyang Tang, Martin B. Dickman, Alan Collmer, James R Alfano

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

The Pseudomonas syringae pv. tomato DC3000 type III secretion system (TTSS) is required for bacterial pathogenicity on plants and elicitation of the hypersensitive response (HR), a programmed cell death (PCD) that occurs on resistant plants. Cosmid pHIR11 enables non-pathogens to elicit an HR dependent upon the TTSS and the effector HopPsyA. We used pHIR11 to determine that effectors HopPtoE, avirulence AvrPphEPto, AvrPpiB1Pto, AvrPtoB, and HopPtoF could suppress a HopPsyA-dependent HR on tobacco and Arabidopsis. Mixed inoculum and Agrobacterium-mediated transient expression experiments confirmed that suppressor action occurred within plant cells. These suppressors, with the exception of AvrPpiB1Pto, inhibited the expression of the tobacco pathogenesis-related (PR) gene PR1a. DC3000 suppressor mutants elicited an enhanced HR consistent with these mutants lacking an HR suppressor. Additionally, HopPtoG was identified as a suppressor on the basis of an enhanced HR produced by a hopPtoG mutant. Remarkably, these proteins functioned to inhibit the ability of the pro-apoptotic protein, Bax to induce PCD in plants and yeast, indicating that these effectors function as anti-PCD proteins in a trans-kingdom manner. The high proportion of effectors that suppress PCD suggests that suppressing plant immunity is one of the primary roles for DC3000 effectors and a central requirement for P. syringae pathogenesis.

Original languageEnglish (US)
Pages (from-to)554-565
Number of pages12
JournalPlant Journal
Volume37
Issue number4
DOIs
StatePublished - Feb 1 2004

Fingerprint

Pseudomonas syringae
hypersensitive response
Apoptosis Regulatory Proteins
Cell Death
apoptosis
Yeasts
Lycopersicon esculentum
yeasts
Tobacco
Plant Immunity
type III secretion system
Cosmids
Agrobacterium
Plant Cells
Arabidopsis
mutants
Virulence
tobacco
pathogenesis
Pseudomonas syringae pv. tomato

Keywords

  • Avr proteins
  • Bacterial plant pathogens
  • Innate immunity
  • Plant defense
  • Programmed cell death
  • Type III effectors

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast. / Jamir, Yashitola; Guo, Ming; Oh, Hye Sook; Petnicki-Ocwieja, Tanja; Chen, Shaorong; Tang, Xiaoyang; Dickman, Martin B.; Collmer, Alan; Alfano, James R.

In: Plant Journal, Vol. 37, No. 4, 01.02.2004, p. 554-565.

Research output: Contribution to journalArticle

Jamir, Y, Guo, M, Oh, HS, Petnicki-Ocwieja, T, Chen, S, Tang, X, Dickman, MB, Collmer, A & Alfano, JR 2004, 'Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast', Plant Journal, vol. 37, no. 4, pp. 554-565. https://doi.org/10.1046/j.1365-313X.2003.01982.x
Jamir, Yashitola ; Guo, Ming ; Oh, Hye Sook ; Petnicki-Ocwieja, Tanja ; Chen, Shaorong ; Tang, Xiaoyang ; Dickman, Martin B. ; Collmer, Alan ; Alfano, James R. / Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast. In: Plant Journal. 2004 ; Vol. 37, No. 4. pp. 554-565.
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AU - Chen, Shaorong

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AU - Dickman, Martin B.

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