Identification of protein kinase C ζ isozyme in hamster pancreas and pancreatic carcinoma cell lines

Satish C. Nair, Parvis M. Pour, Terence Lawson, Carol Kolar, Rick Vaughn, Diane F. Birt

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Abstract

Cellular differentiation and proliferation are dependent upon phosphorylation by endogenous protein kinase C (a) isozymes in many cell types. Western blotting with a C‐terminally directed rabbit polyclonal anti‐PKC ζ antibody detected a doublet of approximately 81 kDa in normal hamster pancreatic tissue and hamster pancreatic carcinoma (PC‐1) and human pancreatic carcinoma (PANC‐1) cells, Preabsorption of the antibody with the specific peptide blocked the appearance of the 81–kDa band, indicating that the band was specifically recognized by the PKC ζ antibody. In contrast, antibodies for PKC α, β, γ, δ, and ϵ failed to show specific immunoreactivity for normal pancreatic tissue or PANC‐1 or PC‐1 cells. Immunocytochemical analysis identified PKC ζ in the cytoplasm of ductules and large ducts, to a lesser extent in the islets of the hamster pancreas, and in the normal cultured pancreatic duct epithelial cells and pancreatic carcinoma (PANC‐1 and PC‐1) cell lines. Specific reactivity was seen by electron microscopy in the ductal cells of the normal pancreatic tissue. In normal pancreatic ductal tissue and primary pancreatic ductal hyperplasia and carcinoma, the proportional labeling of PKC ζ in nuclei and cytoplasm was similar. Our results demonstrating the presence of PKC ζ isozyme in the normal pancreas, cultured normal pancreatic duct epithelial cells, and pancreatic carcinoma cells or carcinoma tissue suggests a role for this isozyme in the normal physiology of the pancreas and perhaps in pancreatic carcinoma. © 1995 Wiley‐ Liss, Inc.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalMolecular Carcinogenesis
Volume14
Issue number3
DOIs
Publication statusPublished - Nov 1995

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Keywords

  • Western blot
  • electron microscopy
  • immunohistochemistry
  • immuno‐gold labeling
  • nuclear localization

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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