Identification of novel human immunodeficiency virus type 1-inhibitory peptides based on the antimicrobial peptide database

Guangshun Wang, Karen M. Watson, Alan Peterkofsky, Robert W. Buckheit

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

To identify novel anti-HIV-1 peptides based on the antimicrobial peptide database (APD; http://aps.unmc.edu/AP/main.php), we have screened 30 candidates and found 11 peptides with 50% effective concentrations (EC50) of <10 μM and therapeutic indices (TI) of up to 17. Furthermore, among the eight peptides (with identical amino acid compositions but different sequences) generated by shuffling the sequence of an aurein 1.2 analog, two had a TI twice that of the original sequence. Because antiviral peptides in the database have an arginine/lysine (R/K) ratio of >1, increases in the Arg contents of amphibian maximin H5 and dermaseptin S9 peptides and the database-derived GLK-19 peptide improved the TIs. These examples demonstrate that the APD is a rich resource and a useful tool for developing novel HIV-1-inhibitory peptides.

Original languageEnglish (US)
Pages (from-to)1343-1346
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume54
Issue number3
DOIs
StatePublished - Mar 1 2010

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ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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