Identification of neural progenitors in the adult mammalian eye

Iqbal Ahmad, Lin Tang, Hao Pham

Research output: Contribution to journalArticle

359 Citations (Scopus)

Abstract

We have shown that the embryonic mammalian retina contains neural progenitors which display stem cell properties in vitro. Here we report the characterization of neural progenitors isolated from the adult mammalian eye. These quiescent cells, located in the pigmented ciliary bodies, proliferate in the presence of FGF2 and express the neuroectodermal marker nestin. The proliferating cells give rise to neural spheres and are multipotential; they express cell type-specific markers corresponding to neurons and glia. In addition, neural progenitors can generate secondary neural spheres, thus displaying potential to self-renew. The ciliary body-derived neural progenitors display retina-specific properties; the undifferentiated cells express Chx10, a retinal progenitor marker, and upon differentiation express markers corresponding to specific retinal cell types. Therefore, the pigmented ciliary body in the adult mammalian eye harbors neural progenitors that display stem cell properties and have the capacity to give rise to retinal neurons in vitro. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)517-521
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number2
DOIs
StatePublished - Apr 13 2000

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Stem cells
Neurons
Ciliary Body
Nestin
Fibroblast Growth Factor 2
Ports and harbors
Cells
Retina
Stem Cells
Retinal Neurons
Differentiation Antigens
Neuroglia
In Vitro Techniques

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of neural progenitors in the adult mammalian eye. / Ahmad, Iqbal; Tang, Lin; Pham, Hao.

In: Biochemical and Biophysical Research Communications, Vol. 270, No. 2, 13.04.2000, p. 517-521.

Research output: Contribution to journalArticle

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