Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method.

Alan R Doster, B. C. Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An indirect immunoperoxidase staining technique was evaluated for detection of Mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. Lungs from swine with induced (n = 4) or naturally occurring M hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against M hyopneumoniae as the primary antibody. Organisms stained by the indirect immunoperoxidase method were identified in tissue sections as pleomorphic brown-staining structures corresponding to those observed with immunofluorescence. Mycoplasma hyosynoviae, M hyorhinis, and Acholeplasma laidlawii did not stain with the indirect immunoperoxidase method, using antibody raised against M hyopneumoniae.

Original languageEnglish (US)
Pages (from-to)1719-1721
Number of pages3
JournalAmerican journal of veterinary research
Volume49
Issue number10
StatePublished - Oct 1988

Fingerprint

Mycoplasma hyopneumoniae
formalin
Formaldehyde
Swine
lungs
Mycoplasma hyosynoviae
Lung
swine
antibodies
Antibodies
Acholeplasma laidlawii
Staining and Labeling
Immunoenzyme Techniques
Paraffin
alkanes
fluorescent antibody technique
Fluorescent Antibody Technique
dyes
Microscopy
microscopy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{d98b488128de455ebe3cf04bc20c3af9,
title = "Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method.",
abstract = "An indirect immunoperoxidase staining technique was evaluated for detection of Mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. Lungs from swine with induced (n = 4) or naturally occurring M hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against M hyopneumoniae as the primary antibody. Organisms stained by the indirect immunoperoxidase method were identified in tissue sections as pleomorphic brown-staining structures corresponding to those observed with immunofluorescence. Mycoplasma hyosynoviae, M hyorhinis, and Acholeplasma laidlawii did not stain with the indirect immunoperoxidase method, using antibody raised against M hyopneumoniae.",
author = "Doster, {Alan R} and Lin, {B. C.}",
year = "1988",
month = "10",
language = "English (US)",
volume = "49",
pages = "1719--1721",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "10",

}

TY - JOUR

T1 - Identification of Mycoplasma hyopneumoniae in formalin-fixed porcine lung, using an indirect immunoperoxidase method.

AU - Doster, Alan R

AU - Lin, B. C.

PY - 1988/10

Y1 - 1988/10

N2 - An indirect immunoperoxidase staining technique was evaluated for detection of Mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. Lungs from swine with induced (n = 4) or naturally occurring M hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against M hyopneumoniae as the primary antibody. Organisms stained by the indirect immunoperoxidase method were identified in tissue sections as pleomorphic brown-staining structures corresponding to those observed with immunofluorescence. Mycoplasma hyosynoviae, M hyorhinis, and Acholeplasma laidlawii did not stain with the indirect immunoperoxidase method, using antibody raised against M hyopneumoniae.

AB - An indirect immunoperoxidase staining technique was evaluated for detection of Mycoplasma hyopneumoniae in formalin-fixed, paraffin-embedded porcine lung. Lungs from swine with induced (n = 4) or naturally occurring M hyopneumoniae infection (n = 31) were examined grossly, by light and immunofluorescent microscopy, and by an indirect immunoperoxidase test, using antibody raised in swine against M hyopneumoniae as the primary antibody. Organisms stained by the indirect immunoperoxidase method were identified in tissue sections as pleomorphic brown-staining structures corresponding to those observed with immunofluorescence. Mycoplasma hyosynoviae, M hyorhinis, and Acholeplasma laidlawii did not stain with the indirect immunoperoxidase method, using antibody raised against M hyopneumoniae.

UR - http://www.scopus.com/inward/record.url?scp=0024094222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024094222&partnerID=8YFLogxK

M3 - Article

C2 - 3056138

AN - SCOPUS:0024094222

VL - 49

SP - 1719

EP - 1721

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 10

ER -