Identification of homogentisate dioxygenase as a target for vitamin E biofortification in oilseeds

Minviluz G. Stacey, Rebecca E. Cahoon, Hanh T. Nguyen, Yaya Cui, Shirley Sato, Cuong T. Nguyen, Nongnat Phoka, Kerry M. Clark, Yan Liang, Joe Forrester, Josef Batek, Phat Tien Do, David A. Sleper, Thomas E. Clemente, Edgar B. Cahoon, Gary Stacey

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Soybean (Glycine max) is a major plant source of protein and oil and produces important secondary metabolites beneficial for human health. As a tool for gene function discovery and improvement of this important crop, a mutant population was generated using fast neutron irradiation. Visual screening of mutagenized seeds identified a mutant line, designated MO12, which produced brown seeds as opposed to the yellow seeds produced by the unmodified Williams 82 parental cultivar. Using forward genetic methods combined with comparative genome hybridization analysis, we were able to establish that deletion of the GmHGO1 gene is the genetic basis of the brown seeded phenotype exhibited by the MO12 mutant line. GmHGO1 encodes a homogentisate dioxygenase (HGO), which catalyzes the committed enzymatic step in homogentisate catabolism. This report describes to our knowledge the first functional characterization of a plant HGO gene, defects of which are linked to the human genetic disease alkaptonuria. We show that reduced homogentisate catabolism in a soybean HGO mutant is an effective strategy for enhancing the production of lipid-soluble antioxidants such as vitamin E, as well as tolerance to herbicides that target pathways associated with homogentisate metabolism. Furthermore, this work demonstrates the utility of fast neutron mutagenesis in identifying novel genes that contribute to soybean agronomic traits.

Original languageEnglish (US)
Pages (from-to)1506-1518
Number of pages13
JournalPlant physiology
Volume172
Issue number3
DOIs
StatePublished - Nov 2016

Fingerprint

Homogentisate 1,2-Dioxygenase
biofortification
oilseeds
Vitamin E
Soybeans
vitamin E
Fast Neutrons
Seeds
mutants
soybeans
metabolism
Alkaptonuria
seeds
comparative genomic hybridization
plant source protein
Plant Proteins
Inborn Genetic Diseases
Comparative Genomic Hybridization
Plant Oils
genes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Stacey, M. G., Cahoon, R. E., Nguyen, H. T., Cui, Y., Sato, S., Nguyen, C. T., ... Stacey, G. (2016). Identification of homogentisate dioxygenase as a target for vitamin E biofortification in oilseeds. Plant physiology, 172(3), 1506-1518. https://doi.org/10.1104/pp.16.00941

Identification of homogentisate dioxygenase as a target for vitamin E biofortification in oilseeds. / Stacey, Minviluz G.; Cahoon, Rebecca E.; Nguyen, Hanh T.; Cui, Yaya; Sato, Shirley; Nguyen, Cuong T.; Phoka, Nongnat; Clark, Kerry M.; Liang, Yan; Forrester, Joe; Batek, Josef; Do, Phat Tien; Sleper, David A.; Clemente, Thomas E.; Cahoon, Edgar B.; Stacey, Gary.

In: Plant physiology, Vol. 172, No. 3, 11.2016, p. 1506-1518.

Research output: Contribution to journalArticle

Stacey, MG, Cahoon, RE, Nguyen, HT, Cui, Y, Sato, S, Nguyen, CT, Phoka, N, Clark, KM, Liang, Y, Forrester, J, Batek, J, Do, PT, Sleper, DA, Clemente, TE, Cahoon, EB & Stacey, G 2016, 'Identification of homogentisate dioxygenase as a target for vitamin E biofortification in oilseeds', Plant physiology, vol. 172, no. 3, pp. 1506-1518. https://doi.org/10.1104/pp.16.00941
Stacey, Minviluz G. ; Cahoon, Rebecca E. ; Nguyen, Hanh T. ; Cui, Yaya ; Sato, Shirley ; Nguyen, Cuong T. ; Phoka, Nongnat ; Clark, Kerry M. ; Liang, Yan ; Forrester, Joe ; Batek, Josef ; Do, Phat Tien ; Sleper, David A. ; Clemente, Thomas E. ; Cahoon, Edgar B. ; Stacey, Gary. / Identification of homogentisate dioxygenase as a target for vitamin E biofortification in oilseeds. In: Plant physiology. 2016 ; Vol. 172, No. 3. pp. 1506-1518.
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