Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry

Mia Nakachi, Midori Matsumoto, Philip M. Terry, Ronald Cerny, Hideaki Moriyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made only after going though strictly defined steps in the fertilization. In sea stars, the fertilization process starts with the chemotaxis of sperm followed by hyperactivation of sperm upon arriving onto the egg coat, and then sperm penetrate to the egg coat before achieving the fusion. To investigate whether the initiation of chemotaxis and the following signaling has species specificity, we conducted comparative studies in the protein level among sea stars, Asterias amurensis, A. forbesi, and Asterina pectinifera. Since transcription of messenger ribonucleic acid (mRNA) has been suppressed in gamete, the roles of sperm proteins during the fertilization cannot be investigated by examining the mRNA profile. Therefore, proteomics analysis by mass spectrometry was used in this study. In sea stars, upon receiving asteroidal sperm-activating peptide (asterosap), the receptor membrane-bound guanylate cyclases in the sperm tail trigger sperm chemotaxis. We confirmed the presence of membrane-bound guanylate cyclases in the three sea star species, and they all had the same structural domains including the extracellular domain, kinase-like domain, and guanylate cyclase domain. The majority of peptides recovered were from α-helices distributed on the solvent side of the protein. More peptides were recovered from the intracellular domains. The transmembrane domain has not been recovered. The functions of the receptors seemed to be conserved among the species. Furthermore, we identified proteins that may be involved in the guanylate cyclase-triggered signaling pathway.

Original languageEnglish (US)
Pages (from-to)564-571
Number of pages8
JournalMarine Biotechnology
Volume10
Issue number5
DOIs
StatePublished - Sep 1 2008

Fingerprint

Starfish
Sperm Tail
guanylate cyclase
Guanylate Cyclase
Asteroidea
sperm
Stars
Mass spectrometry
Spermatozoa
fertilization (reproduction)
Mass Spectrometry
Fertilization
tail
mass spectrometry
spermatozoa
protein
chemotaxis
Chemotaxis
Proteins
peptide

Keywords

  • Asterias amurensis
  • Chemotaxis
  • Guanylate cyclase
  • Proteomics
  • Sea star
  • Sperm

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

Cite this

Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry. / Nakachi, Mia; Matsumoto, Midori; Terry, Philip M.; Cerny, Ronald; Moriyama, Hideaki.

In: Marine Biotechnology, Vol. 10, No. 5, 01.09.2008, p. 564-571.

Research output: Contribution to journalArticle

@article{24ca580adc8249a4b94d0dc12fb37a1e,
title = "Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry",
abstract = "Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made only after going though strictly defined steps in the fertilization. In sea stars, the fertilization process starts with the chemotaxis of sperm followed by hyperactivation of sperm upon arriving onto the egg coat, and then sperm penetrate to the egg coat before achieving the fusion. To investigate whether the initiation of chemotaxis and the following signaling has species specificity, we conducted comparative studies in the protein level among sea stars, Asterias amurensis, A. forbesi, and Asterina pectinifera. Since transcription of messenger ribonucleic acid (mRNA) has been suppressed in gamete, the roles of sperm proteins during the fertilization cannot be investigated by examining the mRNA profile. Therefore, proteomics analysis by mass spectrometry was used in this study. In sea stars, upon receiving asteroidal sperm-activating peptide (asterosap), the receptor membrane-bound guanylate cyclases in the sperm tail trigger sperm chemotaxis. We confirmed the presence of membrane-bound guanylate cyclases in the three sea star species, and they all had the same structural domains including the extracellular domain, kinase-like domain, and guanylate cyclase domain. The majority of peptides recovered were from α-helices distributed on the solvent side of the protein. More peptides were recovered from the intracellular domains. The transmembrane domain has not been recovered. The functions of the receptors seemed to be conserved among the species. Furthermore, we identified proteins that may be involved in the guanylate cyclase-triggered signaling pathway.",
keywords = "Asterias amurensis, Chemotaxis, Guanylate cyclase, Proteomics, Sea star, Sperm",
author = "Mia Nakachi and Midori Matsumoto and Terry, {Philip M.} and Ronald Cerny and Hideaki Moriyama",
year = "2008",
month = "9",
day = "1",
doi = "10.1007/s10126-008-9096-7",
language = "English (US)",
volume = "10",
pages = "564--571",
journal = "Marine Biotechnology",
issn = "1436-2228",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Identification of guanylate cyclases and related signaling proteins in sperm tail from sea stars by mass spectrometry

AU - Nakachi, Mia

AU - Matsumoto, Midori

AU - Terry, Philip M.

AU - Cerny, Ronald

AU - Moriyama, Hideaki

PY - 2008/9/1

Y1 - 2008/9/1

N2 - Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made only after going though strictly defined steps in the fertilization. In sea stars, the fertilization process starts with the chemotaxis of sperm followed by hyperactivation of sperm upon arriving onto the egg coat, and then sperm penetrate to the egg coat before achieving the fusion. To investigate whether the initiation of chemotaxis and the following signaling has species specificity, we conducted comparative studies in the protein level among sea stars, Asterias amurensis, A. forbesi, and Asterina pectinifera. Since transcription of messenger ribonucleic acid (mRNA) has been suppressed in gamete, the roles of sperm proteins during the fertilization cannot be investigated by examining the mRNA profile. Therefore, proteomics analysis by mass spectrometry was used in this study. In sea stars, upon receiving asteroidal sperm-activating peptide (asterosap), the receptor membrane-bound guanylate cyclases in the sperm tail trigger sperm chemotaxis. We confirmed the presence of membrane-bound guanylate cyclases in the three sea star species, and they all had the same structural domains including the extracellular domain, kinase-like domain, and guanylate cyclase domain. The majority of peptides recovered were from α-helices distributed on the solvent side of the protein. More peptides were recovered from the intracellular domains. The transmembrane domain has not been recovered. The functions of the receptors seemed to be conserved among the species. Furthermore, we identified proteins that may be involved in the guanylate cyclase-triggered signaling pathway.

AB - Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made only after going though strictly defined steps in the fertilization. In sea stars, the fertilization process starts with the chemotaxis of sperm followed by hyperactivation of sperm upon arriving onto the egg coat, and then sperm penetrate to the egg coat before achieving the fusion. To investigate whether the initiation of chemotaxis and the following signaling has species specificity, we conducted comparative studies in the protein level among sea stars, Asterias amurensis, A. forbesi, and Asterina pectinifera. Since transcription of messenger ribonucleic acid (mRNA) has been suppressed in gamete, the roles of sperm proteins during the fertilization cannot be investigated by examining the mRNA profile. Therefore, proteomics analysis by mass spectrometry was used in this study. In sea stars, upon receiving asteroidal sperm-activating peptide (asterosap), the receptor membrane-bound guanylate cyclases in the sperm tail trigger sperm chemotaxis. We confirmed the presence of membrane-bound guanylate cyclases in the three sea star species, and they all had the same structural domains including the extracellular domain, kinase-like domain, and guanylate cyclase domain. The majority of peptides recovered were from α-helices distributed on the solvent side of the protein. More peptides were recovered from the intracellular domains. The transmembrane domain has not been recovered. The functions of the receptors seemed to be conserved among the species. Furthermore, we identified proteins that may be involved in the guanylate cyclase-triggered signaling pathway.

KW - Asterias amurensis

KW - Chemotaxis

KW - Guanylate cyclase

KW - Proteomics

KW - Sea star

KW - Sperm

UR - http://www.scopus.com/inward/record.url?scp=49449086805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49449086805&partnerID=8YFLogxK

U2 - 10.1007/s10126-008-9096-7

DO - 10.1007/s10126-008-9096-7

M3 - Article

C2 - 18461395

AN - SCOPUS:49449086805

VL - 10

SP - 564

EP - 571

JO - Marine Biotechnology

JF - Marine Biotechnology

SN - 1436-2228

IS - 5

ER -