Identification of gene products encoded by the latency-related gene of bovine herpesvirus 1

A. Hossain, L. M. Schang, Clinton J Jones

Research output: Contribution to journalArticle

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Abstract

Bovine herpesvirus 1 (BHV-1) establishes a latent infection in sensory ganglionic neurons of infected animals. Expression of latency-related (LR) gene products is controlled by a 980-bp fragment (LR promoter). DNA sequence analysis revealed that two major open reading frames (ORFs) are in the LR gene. Antibodies directed against both ORFs were generated in rabbits by using synthetic peptides. Antibody P2, which is directed to sequences near the amino terminus of ORF 2, recognized a 41-kD a protein in lytically infected cells, suggesting that ORF 2 encodes a protein. When the LR gene was inserted into a mammalian expression vector and subsequently transfected into COS-7 cells, a 41-kDa protein was detected by use of silver-stained sodium dodecyl sulfate-polyacrylamide gels and by the P2 antibody. In contrast, this protein was not detected in mock-transfected cells. Deletion of DNA sequences containing ORF 2 blocked synthesis of the 41-kDa protein in COS-7 cells. Reverse transcriptase-mediated PCRs indicated that splicing occurs near the C terminus of ORF 2. Further studies indicated that LR RNA was alternatively spliced in latently infected cattle and that a fraction of LR RNA was poly(A)+. Taken together, these studies suggested that a spliced LR transcript has the potential to encode a 41-kDa protein.

Original languageEnglish (US)
Pages (from-to)5345-5352
Number of pages8
JournalJournal of virology
Volume69
Issue number9
StatePublished - Jan 1 1995

Fingerprint

Bovine Herpesvirus 1
Bovine herpesvirus 1
Open Reading Frames
open reading frames
Genes
Proteins
genes
proteins
COS Cells
antibodies
Antibodies
RNA
cells
nucleotide sequences
RNA-directed DNA polymerase
sensory neurons
synthetic peptides
alternative splicing
sodium dodecyl sulfate
Sensory Receptor Cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Identification of gene products encoded by the latency-related gene of bovine herpesvirus 1. / Hossain, A.; Schang, L. M.; Jones, Clinton J.

In: Journal of virology, Vol. 69, No. 9, 01.01.1995, p. 5345-5352.

Research output: Contribution to journalArticle

Hossain, A, Schang, LM & Jones, CJ 1995, 'Identification of gene products encoded by the latency-related gene of bovine herpesvirus 1', Journal of virology, vol. 69, no. 9, pp. 5345-5352.
Hossain, A. ; Schang, L. M. ; Jones, Clinton J. / Identification of gene products encoded by the latency-related gene of bovine herpesvirus 1. In: Journal of virology. 1995 ; Vol. 69, No. 9. pp. 5345-5352.
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