Identification of Escherichia coli O157:H7 genomic regions conserved in strains with a genotype associated with human infection

Marina Steele, Kim Ziebell, Yongxiang Zhang, Andrew Benson, Paulina Konczy, Roger Johnson, Victor Gannon

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Beta-glucuronidase-negative, sorbitol-nonfermenting isolates of Shiga toxin-producing Escherichia coli O157 comprise part of a clone complex of related enterohemorrhagic E. coli isolates. High-resolution genotyping shows that the O157 populations have diverged into two different lineages that appear to have different ecologies. To identify genomic regions unique to the most common human-associated genotype, suppression subtractive hybridization was used to identify DNA sequences present in two clinical strains representing the human lineage I O157:H7 strains but absent from two bovine-derived lineage II strains. PCR assays were then used to test for the presence of these regions in 10 lineage I strains and 20 lineage II strains. Twelve conserved regions of genomic difference for lineage I (CRDI) were identified that were each present in at least seven of the lineage I strains but absent in most of the lineage II strains tested. The boundaries of the lineage I conserved regions were further delimited by PCR. Eleven of these CRDI were associated with E. coli Sakal S-loops 14, 16, 69, 72, 78, 82, 83, 91 to 93, 153, and 286, and the final CRDI was located on the pO157 virulence plasmid. Several potential virulence factors were identified within these regions, including a putative hemolysin-activating protein, an iron transport system, and several possible regulatory genes. Cluster analysis based on lineage I conserved regions showed that the presence/absence of these regions was congruent with the inferred phytogeny of the strains.

Original languageEnglish (US)
Pages (from-to)22-31
Number of pages10
JournalApplied and environmental microbiology
Volume73
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Escherichia coli O157
genomics
genotype
Genotype
Enterohemorrhagic Escherichia coli
Shiga-Toxigenic Escherichia coli
Polymerase Chain Reaction
Hemolysin Proteins
Sorbitol
Glucuronidase
Virulence Factors
Protein Transport
Regulator Genes
Infection
Ecology
infection
Cluster Analysis
Virulence
Plasmids
Iron

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Identification of Escherichia coli O157:H7 genomic regions conserved in strains with a genotype associated with human infection. / Steele, Marina; Ziebell, Kim; Zhang, Yongxiang; Benson, Andrew; Konczy, Paulina; Johnson, Roger; Gannon, Victor.

In: Applied and environmental microbiology, Vol. 73, No. 1, 01.01.2007, p. 22-31.

Research output: Contribution to journalArticle

Steele, Marina ; Ziebell, Kim ; Zhang, Yongxiang ; Benson, Andrew ; Konczy, Paulina ; Johnson, Roger ; Gannon, Victor. / Identification of Escherichia coli O157:H7 genomic regions conserved in strains with a genotype associated with human infection. In: Applied and environmental microbiology. 2007 ; Vol. 73, No. 1. pp. 22-31.
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