Identification of DNA sequences in the latency related promoter of bovine herpes virus type I which are bound by neuronal specific factors

Gustavo Delhon, Clinton Jones

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Bovine herpes virus 1 establishes a latent infection in sensory ganglionic neurons of cattle. During a latent infection, latency related (LR) transcripts are the only detectable viral RNAs. DNA sequences in the LR promoter are positively regulated by neural factors. The 5' terminus of LR RNA in productively infected bovine cells is 20-30 nucleotides downstream from two overlapping TATA like elements. In contrast, the major start sites of LR transcription in trigeminal ganglia of latently infected cattle was 200-300 nucleotides upstream. Electrophoretic mobility shift assays (EMSA) were utilized to identify regions of the LR promoter that specifically bind factors present in dorsal root ganglia of cattle. Nuclear extracts from dorsal root ganglia of cattle or rat pheochromocytoma cells (PC12) contain abundant factors which specifically bind to a 72 bp XhoI-XbaI fragment. The 72 bp fragment is adjacent to the major start sites of LR transcriptional in trigeminal ganglia of latently infected cattle. In contrast, nuclear extracts from non-neural cells, bovine turbinate or Rat-2, did not exhibit similar binding patterns suggesting these factors were not abundant, had reduced binding affinity, or were absent in non-neural cells. Binding was localized to a 20 bp region of the XhoI-XbaI fragment by EMSA and Exonuclease III footprinting. When the XhoI-XbaI fragment was deleted, LR promoter activity was repressed in PC12 cells. Taken together, we conclude the XhoI-XbaI fragment is important for LR-RNA expression in neurons.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalVirus Research
Volume51
Issue number1
DOIs
StatePublished - Sep 1 1997

Fingerprint

Viruses
Trigeminal Ganglion
PC12 Cells
Spinal Ganglia
Electrophoretic Mobility Shift Assay
Nucleotides
RNA
Turbinates
Viral RNA
Pheochromocytoma
Sensory Receptor Cells
Infection
Neurons

Keywords

  • Bovine herpes virus 1 latency gene
  • Neuronal specific factors
  • Promoter activation in neurons

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Identification of DNA sequences in the latency related promoter of bovine herpes virus type I which are bound by neuronal specific factors. / Delhon, Gustavo; Jones, Clinton.

In: Virus Research, Vol. 51, No. 1, 01.09.1997, p. 93-103.

Research output: Contribution to journalArticle

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