Identification of differentially expressed genes following treatment of monkey kidney cells with the mycotoxin fumonisin B1

Y. Zhang, C. Jones, M. B. Dickman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Fumonisin B1 (FB1) is a mycotoxin produced by the phytopathogenic fungus Fusarium moniliforme, which structurally resembles sphingoid bases. FB1 perturbs sphingolipid synthesis by inhibiting the activity of ceramide synthase. Depending on the host, ingestion of FB1 causes equine leukoencephalomalacia or porcine pulmonary edema. It is also carcinogenic to rats and may play a role in certain human cancers. Previous studies showed that FB1 repressed specific isoforms of protein kinase C and cyclin-dependent kinase 2 (CDK2) activity. Conversely, FB1 induced expression of CDK inhibitors, p21Waf1/Cip1, p27Kip1, and p57Kip2 in monkey kidney cells (CV-1). Consequently, FB1 treatment of CV-1 cells leads to cell-cycle arrest and apoptosis. The baculovirus IAP gene (inhibitor of apoptosis), which blocks tumor necrosis factor (TNF)-induced apoptosis, protects several fibroblast cell types from apoptosis, suggesting the TNF pathway is important for FB1-induced apoptosis. To identify genes that are induced by FB1, we used a PCR-based subtraction approach. Eight genes that showed high similarity (> 90%) to known mammalian genes were identified. These genes included: tumor necrosis factor type 1 receptor associated protein 2 (TRAP2), human leukemia virus receptor (GLVR1), human Scaffold attachment factor A (SAF-A) also called heterogeneous nuclear ribonucleoprotein U (hnRNP-U), human protein kinase C-binding protein (RACK7), human oligosaccharyl transferase STT3 subunit, mouse WW-domain binding protein 2 (WBP2), human fibronectin, and an unknown human clone. The ability of FB1 to alter gene expression and signal transduction pathways may be necessary for its carcinogenic and toxic effects.

Original languageEnglish (US)
Pages (from-to)45-53
Number of pages9
JournalFood and Chemical Toxicology
Volume39
Issue number1
DOIs
StatePublished - Mar 31 2001

Fingerprint

fumonisin B1
Mycotoxins
kidney cells
mycotoxins
Haplorhini
monkeys
Genes
Kidney
apoptosis
Heterogeneous-Nuclear Ribonucleoprotein U
genes
Apoptosis
tumor necrosis factors
protein kinase C
Protein Kinase C
binding proteins
Tumor Necrosis Factor-alpha
Cells
Cyclin-Dependent Kinase 2
Receptors, Tumor Necrosis Factor, Type I

Keywords

  • Fumonisin B
  • Fusarium moniliforme
  • Mycotoxins
  • PCR-select cDNA subtraction

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Identification of differentially expressed genes following treatment of monkey kidney cells with the mycotoxin fumonisin B1. / Zhang, Y.; Jones, C.; Dickman, M. B.

In: Food and Chemical Toxicology, Vol. 39, No. 1, 31.03.2001, p. 45-53.

Research output: Contribution to journalArticle

@article{373af3963bf54866a468cc7e54ac0a8d,
title = "Identification of differentially expressed genes following treatment of monkey kidney cells with the mycotoxin fumonisin B1",
abstract = "Fumonisin B1 (FB1) is a mycotoxin produced by the phytopathogenic fungus Fusarium moniliforme, which structurally resembles sphingoid bases. FB1 perturbs sphingolipid synthesis by inhibiting the activity of ceramide synthase. Depending on the host, ingestion of FB1 causes equine leukoencephalomalacia or porcine pulmonary edema. It is also carcinogenic to rats and may play a role in certain human cancers. Previous studies showed that FB1 repressed specific isoforms of protein kinase C and cyclin-dependent kinase 2 (CDK2) activity. Conversely, FB1 induced expression of CDK inhibitors, p21Waf1/Cip1, p27Kip1, and p57Kip2 in monkey kidney cells (CV-1). Consequently, FB1 treatment of CV-1 cells leads to cell-cycle arrest and apoptosis. The baculovirus IAP gene (inhibitor of apoptosis), which blocks tumor necrosis factor (TNF)-induced apoptosis, protects several fibroblast cell types from apoptosis, suggesting the TNF pathway is important for FB1-induced apoptosis. To identify genes that are induced by FB1, we used a PCR-based subtraction approach. Eight genes that showed high similarity (> 90{\%}) to known mammalian genes were identified. These genes included: tumor necrosis factor type 1 receptor associated protein 2 (TRAP2), human leukemia virus receptor (GLVR1), human Scaffold attachment factor A (SAF-A) also called heterogeneous nuclear ribonucleoprotein U (hnRNP-U), human protein kinase C-binding protein (RACK7), human oligosaccharyl transferase STT3 subunit, mouse WW-domain binding protein 2 (WBP2), human fibronectin, and an unknown human clone. The ability of FB1 to alter gene expression and signal transduction pathways may be necessary for its carcinogenic and toxic effects.",
keywords = "Fumonisin B, Fusarium moniliforme, Mycotoxins, PCR-select cDNA subtraction",
author = "Y. Zhang and C. Jones and Dickman, {M. B.}",
year = "2001",
month = "3",
day = "31",
doi = "10.1016/S0278-6915(00)00114-9",
language = "English (US)",
volume = "39",
pages = "45--53",
journal = "Food and Chemical Toxicology",
issn = "0278-6915",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Identification of differentially expressed genes following treatment of monkey kidney cells with the mycotoxin fumonisin B1

AU - Zhang, Y.

AU - Jones, C.

AU - Dickman, M. B.

PY - 2001/3/31

Y1 - 2001/3/31

N2 - Fumonisin B1 (FB1) is a mycotoxin produced by the phytopathogenic fungus Fusarium moniliforme, which structurally resembles sphingoid bases. FB1 perturbs sphingolipid synthesis by inhibiting the activity of ceramide synthase. Depending on the host, ingestion of FB1 causes equine leukoencephalomalacia or porcine pulmonary edema. It is also carcinogenic to rats and may play a role in certain human cancers. Previous studies showed that FB1 repressed specific isoforms of protein kinase C and cyclin-dependent kinase 2 (CDK2) activity. Conversely, FB1 induced expression of CDK inhibitors, p21Waf1/Cip1, p27Kip1, and p57Kip2 in monkey kidney cells (CV-1). Consequently, FB1 treatment of CV-1 cells leads to cell-cycle arrest and apoptosis. The baculovirus IAP gene (inhibitor of apoptosis), which blocks tumor necrosis factor (TNF)-induced apoptosis, protects several fibroblast cell types from apoptosis, suggesting the TNF pathway is important for FB1-induced apoptosis. To identify genes that are induced by FB1, we used a PCR-based subtraction approach. Eight genes that showed high similarity (> 90%) to known mammalian genes were identified. These genes included: tumor necrosis factor type 1 receptor associated protein 2 (TRAP2), human leukemia virus receptor (GLVR1), human Scaffold attachment factor A (SAF-A) also called heterogeneous nuclear ribonucleoprotein U (hnRNP-U), human protein kinase C-binding protein (RACK7), human oligosaccharyl transferase STT3 subunit, mouse WW-domain binding protein 2 (WBP2), human fibronectin, and an unknown human clone. The ability of FB1 to alter gene expression and signal transduction pathways may be necessary for its carcinogenic and toxic effects.

AB - Fumonisin B1 (FB1) is a mycotoxin produced by the phytopathogenic fungus Fusarium moniliforme, which structurally resembles sphingoid bases. FB1 perturbs sphingolipid synthesis by inhibiting the activity of ceramide synthase. Depending on the host, ingestion of FB1 causes equine leukoencephalomalacia or porcine pulmonary edema. It is also carcinogenic to rats and may play a role in certain human cancers. Previous studies showed that FB1 repressed specific isoforms of protein kinase C and cyclin-dependent kinase 2 (CDK2) activity. Conversely, FB1 induced expression of CDK inhibitors, p21Waf1/Cip1, p27Kip1, and p57Kip2 in monkey kidney cells (CV-1). Consequently, FB1 treatment of CV-1 cells leads to cell-cycle arrest and apoptosis. The baculovirus IAP gene (inhibitor of apoptosis), which blocks tumor necrosis factor (TNF)-induced apoptosis, protects several fibroblast cell types from apoptosis, suggesting the TNF pathway is important for FB1-induced apoptosis. To identify genes that are induced by FB1, we used a PCR-based subtraction approach. Eight genes that showed high similarity (> 90%) to known mammalian genes were identified. These genes included: tumor necrosis factor type 1 receptor associated protein 2 (TRAP2), human leukemia virus receptor (GLVR1), human Scaffold attachment factor A (SAF-A) also called heterogeneous nuclear ribonucleoprotein U (hnRNP-U), human protein kinase C-binding protein (RACK7), human oligosaccharyl transferase STT3 subunit, mouse WW-domain binding protein 2 (WBP2), human fibronectin, and an unknown human clone. The ability of FB1 to alter gene expression and signal transduction pathways may be necessary for its carcinogenic and toxic effects.

KW - Fumonisin B

KW - Fusarium moniliforme

KW - Mycotoxins

KW - PCR-select cDNA subtraction

UR - http://www.scopus.com/inward/record.url?scp=0035099082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035099082&partnerID=8YFLogxK

U2 - 10.1016/S0278-6915(00)00114-9

DO - 10.1016/S0278-6915(00)00114-9

M3 - Article

C2 - 11259850

AN - SCOPUS:0035099082

VL - 39

SP - 45

EP - 53

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

IS - 1

ER -