Identification of common subpopulations of non-sorbitol-fermenting, β-glucuronidase-negative Escherichia coli O157:H7 from bovine production environments and human clinical samples

Zhijie Yang, Joy Kovar, Jaehyoung Kim, Joseph Nietfeldt, David R. Smith, Rodney A Moxley, Michael E. Olson, Paul D Fey, Andrew K Benson

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Non-sorbitol-fermenting, β-glucuronidase-negative Escherichia coli O157:H7 strains are regarded as a clone complex, and populations from different geographical locations are believed to share a recent common ancestor. Despite their relatedness, high-resolution genotyping methods can detect significant genome variation among different populations. Phylogenetic analysis of high-resolution genotyping data from these strains has shown that subpopulations from geographically unlinked continents can be divided into two primary phylogenetic lineages, termed lineage I and lineage II, and limited studies of the distribution of these lineages suggest there could be differences in their propensity to cause disease in humans or to be transmitted to humans. Because the genotyping methods necessary to discriminate the two lineages are tedious and subjective, these methods are not particularly suited for studying the large sets of strains that are required to systematically evaluate the ecology and transmission characteristics of these lineages. To overcome this limitation, we have developed a lineage-specific polymorphism assay (LSPA) that can readily distinguish between the lineage I and lineage II subpopulations. In the studies reported here, we describe the development of a six-marker test (LSPA-6) and its validation in a side-by-side comparison with octamer-based genome scanning. Analysis of over 1,400 O157:H7 strains with the LSPA-6 demonstrated that five genotypes comprise over 91% of the strains, suggesting that these subpopulations may be widespread.

Original languageEnglish (US)
Pages (from-to)6846-6854
Number of pages9
JournalApplied and environmental microbiology
Volume70
Issue number11
DOIs
StatePublished - Nov 1 2004

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cattle production
Escherichia coli O157
Glucuronidase
subpopulation
polymorphism
assay
genotyping
genome
Genome
phylogenetics
genetic polymorphism
common ancestry
Ecology
relatedness
sampling
assays
Population
clone
genotype
Clone Cells

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Identification of common subpopulations of non-sorbitol-fermenting, β-glucuronidase-negative Escherichia coli O157:H7 from bovine production environments and human clinical samples. / Yang, Zhijie; Kovar, Joy; Kim, Jaehyoung; Nietfeldt, Joseph; Smith, David R.; Moxley, Rodney A; Olson, Michael E.; Fey, Paul D; Benson, Andrew K.

In: Applied and environmental microbiology, Vol. 70, No. 11, 01.11.2004, p. 6846-6854.

Research output: Contribution to journalArticle

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