Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands

Kiyomi Fujikawa-Adachi, Isao Nishimori, Shuji Sakamoto, Masanori Morita, Saburo Onishi, Suguru Yonezawa, Michael A Hollingsworth

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cytoplasmic carbonic anhydrase (CA) II is involved in acid-base balance in a wide variety of tissues. Extracellular CAs, membrane-bound CA IV and excretory CA VI, play a cooperative role with CA II in regulating the luminal pH in kidney and salivary glands, respectively. To extend the evidence into pancreas, we studied messenger RNA (mRNA) expressions of CA IV and CA VI in human pancreas, mRNA expressions of CA II, IV, and VI were studied in human pancreas, kidney, liver, and salivary glands by three different detection methods: reverse transcription-polymerase chain reaction (RT-PCR), RT-PCR- Southern blot, and Northern blot analysis. CA IV mRNA expression was consistently detected in all four tissues except for liver; only RT-PCR- Southern blot successfully identified its expression in liver. In contrast, RTPCR and RT-PCR-Southern blot identified CA VI mRNA in salivary glands and pancreas, but Northern blot failed to detect its expression in pancreas. There was no detectable signal of CA VI expression in kidney and liver by all detection methods. CA II mRNA expression was consistently detected in all tissues studied. These results indicate that pancreas and salivary glands contain both of CA IV and VI and suggest that the extracellular CA isozymes may form a mutually complementary system with CA II to regulate the luminal pH of the pancreatic duct system.

Original languageEnglish (US)
Pages (from-to)329-335
Number of pages7
JournalPancreas
Volume18
Issue number4
DOIs
StatePublished - Jan 1 1999

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Carbonic Anhydrase IV
Carbonic Anhydrase II
Salivary Glands
Pancreas
Messenger RNA
Reverse Transcription
Southern Blotting
Polymerase Chain Reaction
Liver
Kidney
Northern Blotting
Carbonic Anhydrases
Acid-Base Equilibrium
Pancreatic Ducts
Isoenzymes
carbonic anhydrase VI
Membranes

Keywords

  • Carbonic anhydrase
  • Liver
  • MRNA
  • Northern blot
  • Pancreas
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands. / Fujikawa-Adachi, Kiyomi; Nishimori, Isao; Sakamoto, Shuji; Morita, Masanori; Onishi, Saburo; Yonezawa, Suguru; Hollingsworth, Michael A.

In: Pancreas, Vol. 18, No. 4, 01.01.1999, p. 329-335.

Research output: Contribution to journalArticle

Fujikawa-Adachi, K, Nishimori, I, Sakamoto, S, Morita, M, Onishi, S, Yonezawa, S & Hollingsworth, MA 1999, 'Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands', Pancreas, vol. 18, no. 4, pp. 329-335. https://doi.org/10.1097/00006676-199905000-00001
Fujikawa-Adachi K, Nishimori I, Sakamoto S, Morita M, Onishi S, Yonezawa S et al. Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands. Pancreas. 1999 Jan 1;18(4):329-335. https://doi.org/10.1097/00006676-199905000-00001
Fujikawa-Adachi, Kiyomi ; Nishimori, Isao ; Sakamoto, Shuji ; Morita, Masanori ; Onishi, Saburo ; Yonezawa, Suguru ; Hollingsworth, Michael A. / Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands. In: Pancreas. 1999 ; Vol. 18, No. 4. pp. 329-335.
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