Identification of an S-antigen-like molecule in Drosophila melanogaster: An immunohistochemical study

Wolfgang E. Lieb, Laura Smith-Lang, Harminder Singh Dua, Alan C. Christensen, Larry A. Donoso

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Vision is a highly complex process common to many vertebrate and invertebrate species. The visual system of Drosophila represents a valuable model in which to study this process since its chromosomal makeup has been well mapped and many mutants containing specific photoreceptor defects have been described. S-antigen is a well-characterized photoreceptor cell protein which plays a fundamental role in the visual process. In this study, we used a panel of four monoclonal antibodies (MAbs) specific for amino, mid and carboxy terminal amino acid sequences in S-antigen in order to examine S-antigen immunoreactivity in the Drosophila visual system. Two MAbs, MAb 5C6.47 and MAbC10-C10, localized S-antigen to the retinules and ocelli. In contrast, MAbA2-G5 and MAbA9-C6 did not stain. The presence and strong antigenic similarity of an S-antigen homolog in Drosophila suggests that in both vertebrates and invertebrates S-antigen plays a major role in the regulation of the phototransduction of vision.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalExperimental Eye Research
Volume53
Issue number2
DOIs
StatePublished - Aug 1991

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Keywords

  • Drosophila melanogaster
  • S-antigen
  • compound eye
  • immunohistochemistry
  • monoclonal antibody
  • vision

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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