Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses

Erin D. Scully, Teresa Donze-Reiner, Haichuan Wang, Thomas E. Eickhoff, Frederick Baxendale, Paul Twigg, Frank Kovacs, Tiffany Heng-Moss, Scott E. Sattler, Gautam Sarath

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Knowledge of specific peroxidases that respond to aphid herbivory is limited in C4 grasses, but could provide targets for improving defence against these pests. A sorghum (Sorghum bicolor (L.) Moench) peroxidase (SbPrx-1; Sobic.002G416700) has been previously linked to biotic stress responses, and was the starting point for this study. Genomic analyses indicated that SbPrx-1 was part of a clade of five closely related peroxidase genes occurring within a ∼30kb region on chromosome 2 of the sorghum genome. Comparison of this ∼30-kb region to syntenic regions in switchgrass (Panicum virgatum L.) and foxtail millet (Setaria italica L.) identified similar related clusters of peroxidases. Infestation of a susceptible sorghum cultivar with greenbugs (Shizaphis graminum Rondani) induced three of the five peroxidases. Greenbug infestation of switchgrass and foxtail millet plants showed similar inductions of peroxidases. SbPrx-1 was also induced in response to aphid herbivory in a greenbug-resistant sorghum line, Cargill 607E. These data indicate that this genomic region of C4 grasses could be valuable as a marker to assess potential insect resistance in C4 grasses.

Original languageEnglish (US)
Pages (from-to)1134-1148
Number of pages15
JournalFunctional Plant Biology
Volume43
Issue number12
DOIs
StatePublished - Jan 1 2016

Fingerprint

Schizaphis graminum
peroxidases
Sorghum (Poaceae)
Setaria italica
Panicum virgatum
grasses
Aphidoidea
peroxidase
herbivores
genomics
biotic stress
Sorghum bicolor
stress response
pests
chromosomes
insects
genome
cultivars
genes

Keywords

  • defence
  • defense
  • foxtail millet
  • greenbugs
  • plant resistance
  • sorghum
  • switchgrass
  • synteny.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses. / Scully, Erin D.; Donze-Reiner, Teresa; Wang, Haichuan; Eickhoff, Thomas E.; Baxendale, Frederick; Twigg, Paul; Kovacs, Frank; Heng-Moss, Tiffany; Sattler, Scott E.; Sarath, Gautam.

In: Functional Plant Biology, Vol. 43, No. 12, 01.01.2016, p. 1134-1148.

Research output: Contribution to journalArticle

Scully, ED, Donze-Reiner, T, Wang, H, Eickhoff, TE, Baxendale, F, Twigg, P, Kovacs, F, Heng-Moss, T, Sattler, SE & Sarath, G 2016, 'Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses', Functional Plant Biology, vol. 43, no. 12, pp. 1134-1148. https://doi.org/10.1071/FP16104
Scully, Erin D. ; Donze-Reiner, Teresa ; Wang, Haichuan ; Eickhoff, Thomas E. ; Baxendale, Frederick ; Twigg, Paul ; Kovacs, Frank ; Heng-Moss, Tiffany ; Sattler, Scott E. ; Sarath, Gautam. / Identification of an orthologous clade of peroxidases that respond to feeding by greenbugs (Schizaphis graminum) in C4 grasses. In: Functional Plant Biology. 2016 ; Vol. 43, No. 12. pp. 1134-1148.
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