Identification of an NPY-Y1 receptor subtype in bovine chromaffin cells

Peijin Zhang, Jialin C Zheng, Roseann L. Vorce, Terry D. Hexum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bovine chromaffin cells have been used in a variety of studies designed to reveal different aspects of neuropeptide Y (NPY) action. Pharmacological data have defined five NPY receptor subtypes, only one of which (Y3) has not been cloned. Some studies with bovine chromaffin cells have concluded that the effects of NPY on this cell type are mediated by the Y3 subtype. Previous work from our laboratory demonstrates that a Y1 subtype mediates the effect of NPY in this tissue. In the current studies we provide further evidence for the existence of the Y1 subtype in bovine chromaffin cells. BIBP3226, the selective Y1 antagonist, potently displaces [125I]NPY from its binding site IC50=1.91x10-9 M. Moreover, [125I]BIBP3226 binds to bovine chromaffin cell membranes with high affinity (IC50=5.9x10-8 M). Examination of BIBP3226 antagonism of NPY inhibition of forskolin stimulated cyclic AMP accumulation reveals that it is a competitive antagonist with a K(B) similar to the IC50 for [125I]BIBP3226 binding. Northern blot analysis using a porcine cDNA clone for the Y1 subtype demonstrates a 3.5-kb mRNA species in chromaffin cells. These data identify the bovine chromaffin cell NPY receptor as a Y1 subtype.

Original languageEnglish (US)
Pages (from-to)9-13
Number of pages5
JournalRegulatory Peptides
Volume87
Issue number1-3
DOIs
StatePublished - Feb 8 2000

Fingerprint

Chromaffin Cells
Neuropeptide Y
Neuropeptide Y Receptors
Inhibitory Concentration 50
Colforsin
Cell membranes
Cyclic AMP
Northern Blotting
Complementary DNA
Binding Sites
neuropeptide Y-Y1 receptor
Tissue
Swine
Clone Cells
Messenger RNA
Cell Membrane
Pharmacology
BIBP 3226

Keywords

  • Bovine chromaffin cells
  • Neuropeptide Y
  • Receptor subtypes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Identification of an NPY-Y1 receptor subtype in bovine chromaffin cells. / Zhang, Peijin; Zheng, Jialin C; Vorce, Roseann L.; Hexum, Terry D.

In: Regulatory Peptides, Vol. 87, No. 1-3, 08.02.2000, p. 9-13.

Research output: Contribution to journalArticle

Zhang, Peijin ; Zheng, Jialin C ; Vorce, Roseann L. ; Hexum, Terry D. / Identification of an NPY-Y1 receptor subtype in bovine chromaffin cells. In: Regulatory Peptides. 2000 ; Vol. 87, No. 1-3. pp. 9-13.
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