Identification of an IgE-binding region in soybean acidic glycinin G1

Michael G. Zeece, Tom A. Beardslee, John P. Markwell, Gautam Sarath

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immuno-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin G1 was narrowed by in situ digestion with endoproteinase GluC followed by immuno-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.

Original languageEnglish (US)
Pages (from-to)83-90
Number of pages8
JournalFood and Agricultural Immunology
Volume11
Issue number1
DOIs
StatePublished - Mar 1999

Fingerprint

glycinin
Soybeans
Immunoglobulin E
soybeans
immunoblotting
Galectin 3
Peptides
binding proteins
Digestion
polypeptides
digestion
peptides
Serum

Keywords

  • Food allergy
  • Glycinin
  • IgE-binding proteins
  • Soybean

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

Cite this

Identification of an IgE-binding region in soybean acidic glycinin G1. / Zeece, Michael G.; Beardslee, Tom A.; Markwell, John P.; Sarath, Gautam.

In: Food and Agricultural Immunology, Vol. 11, No. 1, 03.1999, p. 83-90.

Research output: Contribution to journalArticle

Zeece, Michael G. ; Beardslee, Tom A. ; Markwell, John P. ; Sarath, Gautam. / Identification of an IgE-binding region in soybean acidic glycinin G1. In: Food and Agricultural Immunology. 1999 ; Vol. 11, No. 1. pp. 83-90.
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