Identification of an epithelial-specific enhancer regulating ESX expression

Richard M. Neve, Hema Parmar, Cliff Amend, Chira Chen, A Angie Rizzino, Christopher C. Benz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The Ets transcription factor, ESX, exhibits a unique pattern of epithelial-restricted expression and transactivates genes involved in epithelial differentiation and cancer. The aim of this study was to determine the underlying genetic basis for epithelial-specific expression of ESX. We have identified a 30bp ESX enhancer sequence (EES) approximately 3 kb upstream of the proximal promoter. This region displays enhancer activity in an epithelial-specific manner and deletion of this region abrogates ESX gene transcription. An EES binding protein complex (EBC) was identified through electrophoretic mobility shift assays whose degree of EES binding correlated well with endogenous ESX levels in epithelial cells and was regulated by epithelial differentiation. Understanding the regulation of this element will lend insight into mechanisms of epithelial differentiation and the etiology of breast cancer and may provide novel targets for cancer therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalGene
Volume367
Issue number1-2
DOIs
Publication statusPublished - Feb 15 2006

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Keywords

  • Breast Cancer
  • Differentiation
  • ELF3
  • ESE1
  • Ets

ASJC Scopus subject areas

  • Genetics

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