Identification of an atypical form of 30 kDa SOD - A possible virulence factor in clinical isolates of Shigella spp

Radhakrishnan Prakash, Halagowder Devaraj, S. Niranjali Devaraj

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Reviews on the pathogenic mechanisms of Shigella species show a lacunae in the understanding of the bacterial antioxidant defense system and its regulations. This study was done to investigate the regulation of expression of antioxidant enzymes in clinical isolates of Shigella species, under various growth conditions. The in vitro expression of superoxide dismutase in the clinical isolates of Shigella spp., is modulated by both endogenous and exogenous factors. During aerobic and iron repleted growth conditions, the expression of the MnSOD and FeSOD enzymes were higher, and an atypical SOD was also expressed. However, under anaerobic growth conditions and in plasmid-cured strains, the antioxidant enzyme activities were decreased and the atypical SOD was not expressed. Absence of the atypical form of SOD may be due to the low oxygen environment. Plasmid-encoded factors may also play a role in the expression of this SOD, which had a molecular weight of ∼30 kDa. In the rat ileal loop ligation assay, mild lesions were observed only in the intestinal microvilli of rats injected with plasmid-cured strains of Shigella spp., suggesting that plasmid-encoded factors, including those that regulate the expression of the atypical SOD, are essential for the virulence of Shigella spp.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalMolecular and cellular biochemistry
Volume267
Issue number1-2
DOIs
StatePublished - Dec 1 2004

Fingerprint

Shigella
Virulence Factors
Plasmids
Antioxidants
Rats
Enzymes
Growth
Enzyme activity
Superoxide Dismutase
Assays
Microvilli
Iron
Molecular weight
Ligation
Virulence
Oxygen
Molecular Weight

Keywords

  • Antioxidant enzymes
  • Iron supplementation
  • Plasmid curing
  • Shigella spp.
  • Superoxide dismutase (SOD)

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Identification of an atypical form of 30 kDa SOD - A possible virulence factor in clinical isolates of Shigella spp. / Prakash, Radhakrishnan; Devaraj, Halagowder; Devaraj, S. Niranjali.

In: Molecular and cellular biochemistry, Vol. 267, No. 1-2, 01.12.2004, p. 91-98.

Research output: Contribution to journalReview article

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