Identification of a very large nuclear estrogen receptor complex

Janina Baranowska-Kortylewicz, Katherine Nelson, Edward J. Pavlik, John R. Van Nagell, Holly H. Gallion, Elvis S. Donaldson, Daniel E. Kenady

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A high mol wt estrogen receptor complex (mol wt: > 669,000) has been isolated from chromatin prepared from mouse uteri with the use of multiple protease inhibitors, exposure to DNAase I, and size exclusion HPLC (SEHPLC). This complex is stable in the presence of molybdate and 0.4 M KC1. Because receptors in the cytosol do not. elute similarly to this large complex when investigated under a wide variety of conditions, it is unlikeiy that random receptor aggregation is responsible for the formation of this nuclear complex. Consequently, this complex may represent a collection of chromatin components that participate in the mechanisms of hormonal regulation. Since the large nuclear receptor complex can be isolated, this hypothesis can be readily subjected to extensive investigation.

Original languageEnglish (US)
Pages (from-to)2189-2191
Number of pages3
JournalEndocrinology
Volume120
Issue number5
DOIs
StatePublished - Jan 1 1987

Fingerprint

Estrogen Receptors
Chromatin
Receptor Aggregation
Deoxyribonuclease I
Cytoplasmic and Nuclear Receptors
Protease Inhibitors
Cytosol
Uterus
High Pressure Liquid Chromatography
molybdate

ASJC Scopus subject areas

  • Endocrinology

Cite this

Baranowska-Kortylewicz, J., Nelson, K., Pavlik, E. J., Van Nagell, J. R., Gallion, H. H., Donaldson, E. S., & Kenady, D. E. (1987). Identification of a very large nuclear estrogen receptor complex. Endocrinology, 120(5), 2189-2191. https://doi.org/10.1210/endo-120-5-2189

Identification of a very large nuclear estrogen receptor complex. / Baranowska-Kortylewicz, Janina; Nelson, Katherine; Pavlik, Edward J.; Van Nagell, John R.; Gallion, Holly H.; Donaldson, Elvis S.; Kenady, Daniel E.

In: Endocrinology, Vol. 120, No. 5, 01.01.1987, p. 2189-2191.

Research output: Contribution to journalArticle

Baranowska-Kortylewicz, J, Nelson, K, Pavlik, EJ, Van Nagell, JR, Gallion, HH, Donaldson, ES & Kenady, DE 1987, 'Identification of a very large nuclear estrogen receptor complex', Endocrinology, vol. 120, no. 5, pp. 2189-2191. https://doi.org/10.1210/endo-120-5-2189
Baranowska-Kortylewicz J, Nelson K, Pavlik EJ, Van Nagell JR, Gallion HH, Donaldson ES et al. Identification of a very large nuclear estrogen receptor complex. Endocrinology. 1987 Jan 1;120(5):2189-2191. https://doi.org/10.1210/endo-120-5-2189
Baranowska-Kortylewicz, Janina ; Nelson, Katherine ; Pavlik, Edward J. ; Van Nagell, John R. ; Gallion, Holly H. ; Donaldson, Elvis S. ; Kenady, Daniel E. / Identification of a very large nuclear estrogen receptor complex. In: Endocrinology. 1987 ; Vol. 120, No. 5. pp. 2189-2191.
@article{28f1b7f9c32946d2b480fc6c974ac0f4,
title = "Identification of a very large nuclear estrogen receptor complex",
abstract = "A high mol wt estrogen receptor complex (mol wt: > 669,000) has been isolated from chromatin prepared from mouse uteri with the use of multiple protease inhibitors, exposure to DNAase I, and size exclusion HPLC (SEHPLC). This complex is stable in the presence of molybdate and 0.4 M KC1. Because receptors in the cytosol do not. elute similarly to this large complex when investigated under a wide variety of conditions, it is unlikeiy that random receptor aggregation is responsible for the formation of this nuclear complex. Consequently, this complex may represent a collection of chromatin components that participate in the mechanisms of hormonal regulation. Since the large nuclear receptor complex can be isolated, this hypothesis can be readily subjected to extensive investigation.",
author = "Janina Baranowska-Kortylewicz and Katherine Nelson and Pavlik, {Edward J.} and {Van Nagell}, {John R.} and Gallion, {Holly H.} and Donaldson, {Elvis S.} and Kenady, {Daniel E.}",
year = "1987",
month = "1",
day = "1",
doi = "10.1210/endo-120-5-2189",
language = "English (US)",
volume = "120",
pages = "2189--2191",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "5",

}

TY - JOUR

T1 - Identification of a very large nuclear estrogen receptor complex

AU - Baranowska-Kortylewicz, Janina

AU - Nelson, Katherine

AU - Pavlik, Edward J.

AU - Van Nagell, John R.

AU - Gallion, Holly H.

AU - Donaldson, Elvis S.

AU - Kenady, Daniel E.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - A high mol wt estrogen receptor complex (mol wt: > 669,000) has been isolated from chromatin prepared from mouse uteri with the use of multiple protease inhibitors, exposure to DNAase I, and size exclusion HPLC (SEHPLC). This complex is stable in the presence of molybdate and 0.4 M KC1. Because receptors in the cytosol do not. elute similarly to this large complex when investigated under a wide variety of conditions, it is unlikeiy that random receptor aggregation is responsible for the formation of this nuclear complex. Consequently, this complex may represent a collection of chromatin components that participate in the mechanisms of hormonal regulation. Since the large nuclear receptor complex can be isolated, this hypothesis can be readily subjected to extensive investigation.

AB - A high mol wt estrogen receptor complex (mol wt: > 669,000) has been isolated from chromatin prepared from mouse uteri with the use of multiple protease inhibitors, exposure to DNAase I, and size exclusion HPLC (SEHPLC). This complex is stable in the presence of molybdate and 0.4 M KC1. Because receptors in the cytosol do not. elute similarly to this large complex when investigated under a wide variety of conditions, it is unlikeiy that random receptor aggregation is responsible for the formation of this nuclear complex. Consequently, this complex may represent a collection of chromatin components that participate in the mechanisms of hormonal regulation. Since the large nuclear receptor complex can be isolated, this hypothesis can be readily subjected to extensive investigation.

UR - http://www.scopus.com/inward/record.url?scp=0023226170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023226170&partnerID=8YFLogxK

U2 - 10.1210/endo-120-5-2189

DO - 10.1210/endo-120-5-2189

M3 - Article

VL - 120

SP - 2189

EP - 2191

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 5

ER -