Identification of a tumor inhibitory factor in rat ascites fluid

Alan E. Levine, David A. Hamilton, Lynn C. Yeoman, Harris Busch, Michael G Brattain

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A polypeptide which inhibits the growth of human carcinoma cells has been characterized from Novikoff rat ascites fluid. This tumor inhibitory factor co-purified with transforming growth factor activity through acid/ethanol extraction and Bio-Gel chromatography. The two activities were completely separated by reverse phase HPLC. The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity. This factor inhibited the anchorage independent growth of the more differentiated human colon carcinoma cell lines but did not affect the less differentiated carcinoma cells. The presence of stimulatory and inhibitory activities in the same extracts suggests that the relative concentrations of these factors may be important in the control of cell growth.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume119
Issue number1
DOIs
StatePublished - Feb 29 1984

Fingerprint

Ascites
Rats
Tumors
Cells
Carcinoma
Fluids
Growth
Neoplasms
Cell growth
Transforming Growth Factors
Chromatography
Bioactivity
Disulfides
Gel Chromatography
Colon
Ethanol
Hot Temperature
Gels
High Pressure Liquid Chromatography
Cell Line

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of a tumor inhibitory factor in rat ascites fluid. / Levine, Alan E.; Hamilton, David A.; Yeoman, Lynn C.; Busch, Harris; Brattain, Michael G.

In: Biochemical and Biophysical Research Communications, Vol. 119, No. 1, 29.02.1984, p. 76-82.

Research output: Contribution to journalArticle

Levine, Alan E. ; Hamilton, David A. ; Yeoman, Lynn C. ; Busch, Harris ; Brattain, Michael G. / Identification of a tumor inhibitory factor in rat ascites fluid. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 119, No. 1. pp. 76-82.
@article{857dfed0202c49c6954b5ea32905fdb7,
title = "Identification of a tumor inhibitory factor in rat ascites fluid",
abstract = "A polypeptide which inhibits the growth of human carcinoma cells has been characterized from Novikoff rat ascites fluid. This tumor inhibitory factor co-purified with transforming growth factor activity through acid/ethanol extraction and Bio-Gel chromatography. The two activities were completely separated by reverse phase HPLC. The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity. This factor inhibited the anchorage independent growth of the more differentiated human colon carcinoma cell lines but did not affect the less differentiated carcinoma cells. The presence of stimulatory and inhibitory activities in the same extracts suggests that the relative concentrations of these factors may be important in the control of cell growth.",
author = "Levine, {Alan E.} and Hamilton, {David A.} and Yeoman, {Lynn C.} and Harris Busch and Brattain, {Michael G}",
year = "1984",
month = "2",
day = "29",
doi = "10.1016/0006-291X(84)91620-6",
language = "English (US)",
volume = "119",
pages = "76--82",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Identification of a tumor inhibitory factor in rat ascites fluid

AU - Levine, Alan E.

AU - Hamilton, David A.

AU - Yeoman, Lynn C.

AU - Busch, Harris

AU - Brattain, Michael G

PY - 1984/2/29

Y1 - 1984/2/29

N2 - A polypeptide which inhibits the growth of human carcinoma cells has been characterized from Novikoff rat ascites fluid. This tumor inhibitory factor co-purified with transforming growth factor activity through acid/ethanol extraction and Bio-Gel chromatography. The two activities were completely separated by reverse phase HPLC. The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity. This factor inhibited the anchorage independent growth of the more differentiated human colon carcinoma cell lines but did not affect the less differentiated carcinoma cells. The presence of stimulatory and inhibitory activities in the same extracts suggests that the relative concentrations of these factors may be important in the control of cell growth.

AB - A polypeptide which inhibits the growth of human carcinoma cells has been characterized from Novikoff rat ascites fluid. This tumor inhibitory factor co-purified with transforming growth factor activity through acid/ethanol extraction and Bio-Gel chromatography. The two activities were completely separated by reverse phase HPLC. The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity. This factor inhibited the anchorage independent growth of the more differentiated human colon carcinoma cell lines but did not affect the less differentiated carcinoma cells. The presence of stimulatory and inhibitory activities in the same extracts suggests that the relative concentrations of these factors may be important in the control of cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0021366939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021366939&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(84)91620-6

DO - 10.1016/0006-291X(84)91620-6

M3 - Article

VL - 119

SP - 76

EP - 82

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -