Identification of a second mimicry epitope from Acanthamoeba castellanii that induces CNS autoimmunity by generating cross-reactive T cells for MBP 89-101 in SJL mice

Chandirasegaran Massilamany, Oluwatoyin A. Asojo, Arunakumar Gangaplara, David Steffen, Jay Reddy

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We had previously reported that Acanthamoeba castellanii (ACA) contains a mimicry epitope for proteolipid protein 139-151 capable of inducing central nervous system (CNS) autoimmunity in SJL/J mice. We now present evidence that ACA also contains a mimicry epitope for myelin basic protein (MBP) 89-101, a derivative from amoebic nicotinamide adenine dinucleotide dehydrogenase subunit 2 (NAD). The epitope, NAD 108-120, contains a discontinuous stretch of six amino acids in the core region (VVFFKNIILIGFL) sharing 46% identity with MBP 89-101 (VHFFKNIVTPRTP; identical residues are underlined). SJL mice immunized with NAD 108-120 develop encephalomyelitis similar to the disease induced by the cognate peptide. We demonstrate that NAD 108-120 induces T cells that cross-react with MBP 89-101; the antigen-sensitized T cells, which produce predominantly T helper (T h) 1 and T h17 cytokines, transfer disease in naive SJL recipients reminiscent of the disease induced with MBP 89-101. This is the first report to demonstrate that a solitary microbe can induce CNS autoimmunity by generating cross-reactive T cells for multiple myelin antigens.

Original languageEnglish (US)
Pages (from-to)729-739
Number of pages11
JournalInternational Immunology
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2011

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Keywords

  • Acanthamoeba castellanii
  • Experimental autoimmune encephalomyelitis
  • Molecular mimicry
  • Multiple sclerosis
  • Myelin basic protein

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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