Identification of a novel herpes simplex virus type 1 transcript and protein (AL3) expressed during latency

Tareq Jaber, Gail Henderson, Sumin Li, Guey Chuen Perng, Dale Carpenter, Steven L. Wechsler, Clinton J Jones

Research output: Contribution to journalArticle

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Abstract

The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) is abundantly expressed in latently infected sensory neurons. In small animal models of infection, expression of the first 1.5 kb of LAT coding sequences is necessary and sufficient for wild-type reactivation from latency. The ability of LAT to inhibit apoptosis is important for reactivation from latency. Within the first 1.5 kb of LAT coding sequences and LAT promoter sequences, additional transcripts have been identified. For example, the anti-sense to LAT transcript (AL) is expressed in the opposite direction to LAT from the 5′ end of LAT and LAT promoter sequences. In addition, the upstream of LAT (UOL) transcript is expressed in the LAT direction from sequences in the LAT promoter. Further examination of the first 1.5 kb of LAT coding sequences revealed two small ORFs that are anti-sense with respect to LAT (AL2 and AL3). A transcript spanning AL3 was detected in productively infected cells, mouse neuroblastoma cells stably expressing LAT and trigeminal ganglia (TG) of latently infected mice. Peptide-specific IgG directed against AL3 specifically recognized a protein migrating near 15 kDa in cells stably transfected with LAT, mouse neuroblastoma cells transfected with a plasmid containing the AL3 ORF and TG of latently infected mice. The inability to detect the AL3 protein during productive infection may have been because the 5′ terminus of the AL3 transcript was downstream of the first in-frame methionine of the AL3 ORF during productive infection.

Original languageEnglish (US)
Pages (from-to)2342-2352
Number of pages11
JournalJournal of General Virology
Volume90
Issue number10
DOIs
StatePublished - Nov 9 2009

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Human Herpesvirus 1
Open Reading Frames
Trigeminal Ganglion
Neuroblastoma
Proteins
Infection
Sensory Receptor Cells
Methionine
Plasmids
Animal Models
Immunoglobulin G
Apoptosis
Peptides
Direction compound

ASJC Scopus subject areas

  • Virology

Cite this

Jaber, T., Henderson, G., Li, S., Perng, G. C., Carpenter, D., Wechsler, S. L., & Jones, C. J. (2009). Identification of a novel herpes simplex virus type 1 transcript and protein (AL3) expressed during latency. Journal of General Virology, 90(10), 2342-2352. https://doi.org/10.1099/vir.0.013318-0

Identification of a novel herpes simplex virus type 1 transcript and protein (AL3) expressed during latency. / Jaber, Tareq; Henderson, Gail; Li, Sumin; Perng, Guey Chuen; Carpenter, Dale; Wechsler, Steven L.; Jones, Clinton J.

In: Journal of General Virology, Vol. 90, No. 10, 09.11.2009, p. 2342-2352.

Research output: Contribution to journalArticle

Jaber, T, Henderson, G, Li, S, Perng, GC, Carpenter, D, Wechsler, SL & Jones, CJ 2009, 'Identification of a novel herpes simplex virus type 1 transcript and protein (AL3) expressed during latency', Journal of General Virology, vol. 90, no. 10, pp. 2342-2352. https://doi.org/10.1099/vir.0.013318-0
Jaber, Tareq ; Henderson, Gail ; Li, Sumin ; Perng, Guey Chuen ; Carpenter, Dale ; Wechsler, Steven L. ; Jones, Clinton J. / Identification of a novel herpes simplex virus type 1 transcript and protein (AL3) expressed during latency. In: Journal of General Virology. 2009 ; Vol. 90, No. 10. pp. 2342-2352.
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