Identification of a novel family of 70 kDa microtubule-associated proteins in Arabidopsis cells

Andrey V. Korolev, Jordi Chan, Mike J. Naldrett, John H. Doonan, Clive W. Lloyd

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Most plant microtubule-associated proteins (MAPs) have homologues across the phylogenetic spectrum. To find potential plant-specific MAPs that will have evaded bioinformatic searches we devised a low stringency method for isolating proteins from an Arabidopsis cell suspension on endogenous taxol-microtubules. By tryptic peptide mass fingerprinting we identified 55 proteins that were enriched on taxol-microtubules. Amongst a range of known MAPs, such as kinesins, MAP65 isoforms and MOR1, we detected 'unknown' 70 kDa proteins that belong to a family of five closely related Arabidopsis proteins having no known homologues amongst non-plant organisms. To verify that AtMAP70-1 associates with microtubules in vivo, it was expressed as a GFP fusion. This confirmed that the protein decorates all four microtubule arrays in both transiently infected Arabidopsis and stably transformed tobacco BY-2 suspension cells. Microtubule-directed drugs perturbed the localization of AtMAP70-1 but cytochalasin D did not. AtMAP70-1 contains four predicted coiled-coil domains and truncation studies identified a central domain that targets the fusion protein to microtubules in vivo. This study therefore introduces a novel family of plant-specific proteins that interact with microtubules.

Original languageEnglish (US)
Pages (from-to)547-555
Number of pages9
JournalPlant Journal
Volume42
Issue number4
DOIs
StatePublished - May 1 2005

Fingerprint

Microtubule-Associated Proteins
Arabidopsis
Microtubules
microtubules
Arabidopsis Proteins
Plant Proteins
proteins
cells
Paclitaxel
Suspensions
paclitaxel
Microtubule Proteins
Cytochalasin D
Kinesin
Proteins
Peptide Mapping
cell suspension culture
Computational Biology
Tobacco
Protein Isoforms

Keywords

  • Arabidopsis
  • GATEWAY
  • Microtubule-associated proteins
  • Proteomics

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Identification of a novel family of 70 kDa microtubule-associated proteins in Arabidopsis cells. / Korolev, Andrey V.; Chan, Jordi; Naldrett, Mike J.; Doonan, John H.; Lloyd, Clive W.

In: Plant Journal, Vol. 42, No. 4, 01.05.2005, p. 547-555.

Research output: Contribution to journalArticle

Korolev, Andrey V. ; Chan, Jordi ; Naldrett, Mike J. ; Doonan, John H. ; Lloyd, Clive W. / Identification of a novel family of 70 kDa microtubule-associated proteins in Arabidopsis cells. In: Plant Journal. 2005 ; Vol. 42, No. 4. pp. 547-555.
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