Identification of a glutamate / aspartate transporter in the rat cochlea

Ha Sheng Li, Andrew S. Niedzielski, Kirk W. Beisel, Hakim Hiel, Robert J. Wenthold, Barbara J Morley

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The neurotransmitter at the synapses between hair cells and spiral ganglion cells in the cochlea is probably L-glutamate or a similar excitatory amino acid. Glutamate uptake by nerve terminals and glial cells is an important component of neurotransmission at glutamatergic synapses of the central nervous system, for providing a reservoir of transmitter or transmitter precursors and the termination of the released glutamate. Hair cell synapses are not surrounded by glial cells, therefore, the uptake mechanism for glutamate in the cochlea may be unique. cDNA was synthesized from total RNA isolated separately from the rat organ of Corti, spiral ganglia, and lateral wall tissues. The expression of a glutamate/aspartate transporter (GLAST) was detected by DNA amplification with the polymerase chain reaction. The other two members of glutamate transporters in this family were not detected by this method. A partial cDNA encoding to GLAST was identified by sequence analysis in a rat cochlear cDNA library. Data concerning the expression and the molecular structure of the glutamate transporter GLAST in the cochlea may provide important information regarding the neurotransmission process at the hair cell-afferent synapses.

Original languageEnglish (US)
Pages (from-to)235-242
Number of pages8
JournalHearing Research
Volume78
Issue number2
DOIs
StatePublished - Aug 1994

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Amino Acid Transport System X-AG
Cochlea
Synapses
Glutamic Acid
Spiral Ganglion
Synaptic Transmission
Neuroglia
Complementary DNA
Organ of Corti
Excitatory Amino Acids
Molecular Structure
Gene Library
Neurotransmitter Agents
Sequence Analysis
Central Nervous System
RNA
Neurons
Polymerase Chain Reaction
DNA

Keywords

  • Cochlea
  • Glutamate transporter
  • Polymerase chain reaction
  • cDNA

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Li, H. S., Niedzielski, A. S., Beisel, K. W., Hiel, H., Wenthold, R. J., & Morley, B. J. (1994). Identification of a glutamate / aspartate transporter in the rat cochlea. Hearing Research, 78(2), 235-242. https://doi.org/10.1016/0378-5955(94)90029-9

Identification of a glutamate / aspartate transporter in the rat cochlea. / Li, Ha Sheng; Niedzielski, Andrew S.; Beisel, Kirk W.; Hiel, Hakim; Wenthold, Robert J.; Morley, Barbara J.

In: Hearing Research, Vol. 78, No. 2, 08.1994, p. 235-242.

Research output: Contribution to journalArticle

Li, HS, Niedzielski, AS, Beisel, KW, Hiel, H, Wenthold, RJ & Morley, BJ 1994, 'Identification of a glutamate / aspartate transporter in the rat cochlea', Hearing Research, vol. 78, no. 2, pp. 235-242. https://doi.org/10.1016/0378-5955(94)90029-9
Li, Ha Sheng ; Niedzielski, Andrew S. ; Beisel, Kirk W. ; Hiel, Hakim ; Wenthold, Robert J. ; Morley, Barbara J. / Identification of a glutamate / aspartate transporter in the rat cochlea. In: Hearing Research. 1994 ; Vol. 78, No. 2. pp. 235-242.
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