Identification and quantitation of 7-(Benzo[a-]pyren-6-yl)guanine in the urine and feces of rats treated with benzo[a]pyrene

Eleanor G Rogan, N. V S RamaKrishna, Sheila Higginbotham, Ercole Cavalieri, Hyuk Jeong, Ryszard Jankowiak, Gerald J. Small

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The major identified benzo[o]pyrene (BP)-DNA adduct formed by cytochrome P-450 contains BP bound at the C-6 position to the N-7 position of guanine (BP-N7Gua). This adduct is rapidly depurinated from DNA. When rats were treated with [14C]BP, about 0.02% of the administered dose of BP was excreted as BP-N7Gua in feces and urine within 5 days. Chloroform extracts of the urine and feces were analyzed by high-pressure liquid chromatography. The structure of the adduct was established by cochromatography with electrochemically prepared BP-N7Gua and by fluorescence line narrowing spectrometry. This study represents the first demonstration that BP-N7Gua is formed in vivo in animals treated with BP.

Original languageEnglish (US)
Pages (from-to)441-444
Number of pages4
JournalChemical Research in Toxicology
Volume3
Issue number5
DOIs
StatePublished - Jan 1 1990

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High pressure liquid chromatography
DNA Adducts
Benzo(a)pyrene
Guanine
Chloroform
Feces
Cytochrome P-450 Enzyme System
Spectrometry
Rats
Animals
Demonstrations
Fluorescence
Urine
DNA
Spectrum Analysis
High Pressure Liquid Chromatography
pyrene
7-(benzo(a)pyren-6-yl)guanine

ASJC Scopus subject areas

  • Toxicology

Cite this

Identification and quantitation of 7-(Benzo[a-]pyren-6-yl)guanine in the urine and feces of rats treated with benzo[a]pyrene. / Rogan, Eleanor G; RamaKrishna, N. V S; Higginbotham, Sheila; Cavalieri, Ercole; Jeong, Hyuk; Jankowiak, Ryszard; Small, Gerald J.

In: Chemical Research in Toxicology, Vol. 3, No. 5, 01.01.1990, p. 441-444.

Research output: Contribution to journalArticle

Rogan, Eleanor G ; RamaKrishna, N. V S ; Higginbotham, Sheila ; Cavalieri, Ercole ; Jeong, Hyuk ; Jankowiak, Ryszard ; Small, Gerald J. / Identification and quantitation of 7-(Benzo[a-]pyren-6-yl)guanine in the urine and feces of rats treated with benzo[a]pyrene. In: Chemical Research in Toxicology. 1990 ; Vol. 3, No. 5. pp. 441-444.
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abstract = "The major identified benzo[o]pyrene (BP)-DNA adduct formed by cytochrome P-450 contains BP bound at the C-6 position to the N-7 position of guanine (BP-N7Gua). This adduct is rapidly depurinated from DNA. When rats were treated with [14C]BP, about 0.02{\%} of the administered dose of BP was excreted as BP-N7Gua in feces and urine within 5 days. Chloroform extracts of the urine and feces were analyzed by high-pressure liquid chromatography. The structure of the adduct was established by cochromatography with electrochemically prepared BP-N7Gua and by fluorescence line narrowing spectrometry. This study represents the first demonstration that BP-N7Gua is formed in vivo in animals treated with BP.",
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AU - Jankowiak, Ryszard

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