Identification and localization of a novel zinc finger gene in developing chick skin and feather buds

Babu J. Padanilam, Michael Solursh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have cloned and sequenced a cDNA encoding a novel zinc finger protein (Fzf-1) containing two tandem repeats of zinc finger motifs of the C2H2 type. The cDNA is 3.0 Kb long and has an open reading frame which codes for a protein of 789 amino acids. The expression pattern of the zinc finger gene was studied in chick embryonic skin and feathers by in situ hybridization. The expression of the gene is found to be temporally and spatially regulated. In stage 38 chick embryos, the transcripts are localized to the epidermis but in 10-day-old embryos, the signal is localized to the forming dermis. In 12-day-old chick, the transcripts are localized to the mesenchymal region of the elongated feather buds. Reverse transcription followed by Polymerase Chain Reaction (RT-PCR) did not detect the transcripts in any other tissues.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume220
Issue number1
DOIs
StatePublished - Mar 7 1996

Fingerprint

Feathers
Zinc Fingers
Zinc
Skin
Genes
Complementary DNA
Tandem Repeat Sequences
Polymerase chain reaction
Chick Embryo
Transcription
Dermis
Epidermis
Open Reading Frames
Reverse Transcription
In Situ Hybridization
Proteins
Embryonic Structures
Tissue
Gene Expression
Amino Acids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and localization of a novel zinc finger gene in developing chick skin and feather buds. / Padanilam, Babu J.; Solursh, Michael.

In: Biochemical and Biophysical Research Communications, Vol. 220, No. 1, 07.03.1996, p. 63-67.

Research output: Contribution to journalArticle

@article{aab2c29efe01478b90340b33d8ba4724,
title = "Identification and localization of a novel zinc finger gene in developing chick skin and feather buds",
abstract = "We have cloned and sequenced a cDNA encoding a novel zinc finger protein (Fzf-1) containing two tandem repeats of zinc finger motifs of the C2H2 type. The cDNA is 3.0 Kb long and has an open reading frame which codes for a protein of 789 amino acids. The expression pattern of the zinc finger gene was studied in chick embryonic skin and feathers by in situ hybridization. The expression of the gene is found to be temporally and spatially regulated. In stage 38 chick embryos, the transcripts are localized to the epidermis but in 10-day-old embryos, the signal is localized to the forming dermis. In 12-day-old chick, the transcripts are localized to the mesenchymal region of the elongated feather buds. Reverse transcription followed by Polymerase Chain Reaction (RT-PCR) did not detect the transcripts in any other tissues.",
author = "Padanilam, {Babu J.} and Michael Solursh",
year = "1996",
month = "3",
day = "7",
doi = "10.1006/bbrc.1996.0357",
language = "English (US)",
volume = "220",
pages = "63--67",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Identification and localization of a novel zinc finger gene in developing chick skin and feather buds

AU - Padanilam, Babu J.

AU - Solursh, Michael

PY - 1996/3/7

Y1 - 1996/3/7

N2 - We have cloned and sequenced a cDNA encoding a novel zinc finger protein (Fzf-1) containing two tandem repeats of zinc finger motifs of the C2H2 type. The cDNA is 3.0 Kb long and has an open reading frame which codes for a protein of 789 amino acids. The expression pattern of the zinc finger gene was studied in chick embryonic skin and feathers by in situ hybridization. The expression of the gene is found to be temporally and spatially regulated. In stage 38 chick embryos, the transcripts are localized to the epidermis but in 10-day-old embryos, the signal is localized to the forming dermis. In 12-day-old chick, the transcripts are localized to the mesenchymal region of the elongated feather buds. Reverse transcription followed by Polymerase Chain Reaction (RT-PCR) did not detect the transcripts in any other tissues.

AB - We have cloned and sequenced a cDNA encoding a novel zinc finger protein (Fzf-1) containing two tandem repeats of zinc finger motifs of the C2H2 type. The cDNA is 3.0 Kb long and has an open reading frame which codes for a protein of 789 amino acids. The expression pattern of the zinc finger gene was studied in chick embryonic skin and feathers by in situ hybridization. The expression of the gene is found to be temporally and spatially regulated. In stage 38 chick embryos, the transcripts are localized to the epidermis but in 10-day-old embryos, the signal is localized to the forming dermis. In 12-day-old chick, the transcripts are localized to the mesenchymal region of the elongated feather buds. Reverse transcription followed by Polymerase Chain Reaction (RT-PCR) did not detect the transcripts in any other tissues.

UR - http://www.scopus.com/inward/record.url?scp=0029917499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029917499&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.0357

DO - 10.1006/bbrc.1996.0357

M3 - Article

C2 - 8602858

AN - SCOPUS:0029917499

VL - 220

SP - 63

EP - 67

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -