Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein

Chung Yip Chan, Mohammad R. Salabat, Xian Zhong Ding, David L. Kelly, Mark S. Talamonti, Richard H. Bell, Thomas E. Adrian

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

Original languageEnglish (US)
Pages (from-to)755-764
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number2
DOIs
StatePublished - Apr 8 2005

Fingerprint

Tetradecanoylphorbol Acetate
Computer Simulation
Membrane Proteins
Acetates
Genes
Microarrays
Up-Regulation
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Luciferases
Pancreatic Neoplasms
HeLa Cells
Oligonucleotides
Endoplasmic Reticulum
Carcinogens
Names
Real-Time Polymerase Chain Reaction
Assays
Cells
Cell Line

Keywords

  • 12-O-Tetradecanoylphorbol-13-acetate
  • In silico analysis
  • Luciferase assay
  • Microarray
  • Novel protein
  • Protein kinase C
  • Real-time PCR
  • TTMP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification and in silico characterization of a novel gene : TPA induced trans-membrane protein. / Chan, Chung Yip; Salabat, Mohammad R.; Ding, Xian Zhong; Kelly, David L.; Talamonti, Mark S.; Bell, Richard H.; Adrian, Thomas E.

In: Biochemical and Biophysical Research Communications, Vol. 329, No. 2, 08.04.2005, p. 755-764.

Research output: Contribution to journalArticle

Chan, Chung Yip ; Salabat, Mohammad R. ; Ding, Xian Zhong ; Kelly, David L. ; Talamonti, Mark S. ; Bell, Richard H. ; Adrian, Thomas E. / Identification and in silico characterization of a novel gene : TPA induced trans-membrane protein. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 329, No. 2. pp. 755-764.
@article{3dbfcfa89ba746c9abad196935c345e6,
title = "Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein",
abstract = "12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.",
keywords = "12-O-Tetradecanoylphorbol-13-acetate, In silico analysis, Luciferase assay, Microarray, Novel protein, Protein kinase C, Real-time PCR, TTMP",
author = "Chan, {Chung Yip} and Salabat, {Mohammad R.} and Ding, {Xian Zhong} and Kelly, {David L.} and Talamonti, {Mark S.} and Bell, {Richard H.} and Adrian, {Thomas E.}",
year = "2005",
month = "4",
day = "8",
doi = "10.1016/j.bbrc.2005.02.037",
language = "English (US)",
volume = "329",
pages = "755--764",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Identification and in silico characterization of a novel gene

T2 - TPA induced trans-membrane protein

AU - Chan, Chung Yip

AU - Salabat, Mohammad R.

AU - Ding, Xian Zhong

AU - Kelly, David L.

AU - Talamonti, Mark S.

AU - Bell, Richard H.

AU - Adrian, Thomas E.

PY - 2005/4/8

Y1 - 2005/4/8

N2 - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

AB - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

KW - 12-O-Tetradecanoylphorbol-13-acetate

KW - In silico analysis

KW - Luciferase assay

KW - Microarray

KW - Novel protein

KW - Protein kinase C

KW - Real-time PCR

KW - TTMP

UR - http://www.scopus.com/inward/record.url?scp=14644388201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644388201&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.02.037

DO - 10.1016/j.bbrc.2005.02.037

M3 - Article

C2 - 15737651

AN - SCOPUS:14644388201

VL - 329

SP - 755

EP - 764

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -