Identification and in silico characterization of a novel gene: TPA induced trans-membrane protein

Chung Yip Chan, Mohammad R. Salabat, Xian Zhong Ding, David L. Kelly, Mark S. Talamonti, Richard H. Bell, Thomas E. Adrian

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter with wide ranging, diverse, and sometimes opposite cellular effects. Using oligonucleotide microarray analysis, we have identified a novel gene that is upregulated following treatment with TPA in the pancreatic cancer cell line CD18. Real-time PCR validated the microarray results in CD18 and HeLa cells, and showed that upregulation of the gene is time- and concentration-dependent. In silico analysis showed the gene product to be a single-pass transmembrane protein of 217 residues that is localized to the endoplasmic reticulum, thus the name TPA induced trans-membrane protein (TTMP). A luciferase reporter assay demonstrated that upregulation of TTMP by TPA is triggered at the promoter level.

Original languageEnglish (US)
Pages (from-to)755-764
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number2
DOIs
Publication statusPublished - Apr 8 2005

    Fingerprint

Keywords

  • 12-O-Tetradecanoylphorbol-13-acetate
  • In silico analysis
  • Luciferase assay
  • Microarray
  • Novel protein
  • Protein kinase C
  • Real-time PCR
  • TTMP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this