Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51

Dmitri Fomenko, E. I. Basyuk, V. M. Bezrukov, A. A. Volodin, A. Z. Metlitskaya, I. A. Khmel'

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A structural gene of heptapeptide, which is a component of the microcin C51 molecule, was identified by hybridization of plasmid DNA fragments and a mixture of synthesized oligonucleotides with the sequence corresponding to that of the amino acids in the peptide. Sequence analysis of the structural gene of microcin peptide and its promoter region was performed. The data obtained indicate that the peptide of microcin C51 is synthesized on ribosomes. Four polypeptides of 67, 39, 16, and 14 kDa were identified using the system of minicells. These polypeptides are specified by a DNA fragment responsible for microcin synthesis and immunity in a producer cell. Apparently, three of these polypeptides with molecular masses of 67, 39, and 16 kDa are associated with microcin production and immunity. The 67 kDa polypeptide is involved in the expression of immunity to microcin and, probably, in microcin production.

Original languageEnglish (US)
Pages (from-to)1149-1155
Number of pages7
JournalRussian Journal of Genetics
Volume32
Issue number10
StatePublished - Oct 1 1996

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Plasmids
Gene Expression
Peptides
Immunity
DNA
microcin
Ribosomes
Genetic Promoter Regions
Oligonucleotides
Genes
Sequence Analysis
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Fomenko, D., Basyuk, E. I., Bezrukov, V. M., Volodin, A. A., Metlitskaya, A. Z., & Khmel', I. A. (1996). Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51. Russian Journal of Genetics, 32(10), 1149-1155.

Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51. / Fomenko, Dmitri; Basyuk, E. I.; Bezrukov, V. M.; Volodin, A. A.; Metlitskaya, A. Z.; Khmel', I. A.

In: Russian Journal of Genetics, Vol. 32, No. 10, 01.10.1996, p. 1149-1155.

Research output: Contribution to journalArticle

Fomenko, D, Basyuk, EI, Bezrukov, VM, Volodin, AA, Metlitskaya, AZ & Khmel', IA 1996, 'Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51', Russian Journal of Genetics, vol. 32, no. 10, pp. 1149-1155.
Fomenko D, Basyuk EI, Bezrukov VM, Volodin AA, Metlitskaya AZ, Khmel' IA. Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51. Russian Journal of Genetics. 1996 Oct 1;32(10):1149-1155.
Fomenko, Dmitri ; Basyuk, E. I. ; Bezrukov, V. M. ; Volodin, A. A. ; Metlitskaya, A. Z. ; Khmel', I. A. / Identification and Expression of Plasmid Genes Involved in Synthesis of Microcin C51. In: Russian Journal of Genetics. 1996 ; Vol. 32, No. 10. pp. 1149-1155.
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