Identification and expression of plasmid genes involved in synthesis of microcin C51

D. E. Fomenko, E. I. Basyuk, V. M. Bezrukov, A. A. Volodin, A. Z. Metlitskaya, I. A. Khmef

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A structural gene of heptapeptide, which is a component of the microcin C51 molecule, was identified by hybridization of plasmid DNA fragments and a mixture of synthesized oligonucleotides with the sequence corresponding to that of amino acids in the peptide. Sequence analysis of the structural gene of microcin peptide and its promoter region was performed. The data obtained indicate that the peptide of microcin C51 is synthesized on ribosomes. Four polypeptides of 67, 39, 16, and 14 kDa were identified using the system of minicells. These polypeptides are specified by a DNA fragment responsible for microcin synthesis and immunity in a producer cell. Apparently, three of these polypeptides with molecular masses of 67, 39, and 16 kDa are responsible for microcin production and immunity. The 67 kDa polypeptide is involved in the expression of immunity to microcin and, probably, in microcin production.

Original languageEnglish (US)
Pages (from-to)1326-1332
Number of pages7
JournalGenetika
Volume32
Issue number10
StatePublished - Dec 1 1996

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Plasmids
Gene Expression
Peptides
Immunity
DNA
microcin
Ribosomes
Genetic Promoter Regions
Oligonucleotides
Genes
Sequence Analysis
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fomenko, D. E., Basyuk, E. I., Bezrukov, V. M., Volodin, A. A., Metlitskaya, A. Z., & Khmef, I. A. (1996). Identification and expression of plasmid genes involved in synthesis of microcin C51. Genetika, 32(10), 1326-1332.

Identification and expression of plasmid genes involved in synthesis of microcin C51. / Fomenko, D. E.; Basyuk, E. I.; Bezrukov, V. M.; Volodin, A. A.; Metlitskaya, A. Z.; Khmef, I. A.

In: Genetika, Vol. 32, No. 10, 01.12.1996, p. 1326-1332.

Research output: Contribution to journalArticle

Fomenko, DE, Basyuk, EI, Bezrukov, VM, Volodin, AA, Metlitskaya, AZ & Khmef, IA 1996, 'Identification and expression of plasmid genes involved in synthesis of microcin C51', Genetika, vol. 32, no. 10, pp. 1326-1332.
Fomenko DE, Basyuk EI, Bezrukov VM, Volodin AA, Metlitskaya AZ, Khmef IA. Identification and expression of plasmid genes involved in synthesis of microcin C51. Genetika. 1996 Dec 1;32(10):1326-1332.
Fomenko, D. E. ; Basyuk, E. I. ; Bezrukov, V. M. ; Volodin, A. A. ; Metlitskaya, A. Z. ; Khmef, I. A. / Identification and expression of plasmid genes involved in synthesis of microcin C51. In: Genetika. 1996 ; Vol. 32, No. 10. pp. 1326-1332.
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