Identification and characterization of the bovine immunodeficiency-like virus tat gene

Z. Q. Liu, D. Sheridan, C. Wood

Research output: Contribution to journalComment/debate

27 Citations (Scopus)

Abstract

A cDNA clone of the bovine immunodeficiency-like virus (BIV) trans- activator gene (tat) was identified and characterized. The tat cDNA clone was generated by splicing, and on the basis of sequence analysis, the Tat protein was found to be encoded entirely by the first exon. It is 103 amino acids in size and shares sequence homology with the human immunodeficiency virus (HIV) Tat. The BIV tat clone can trans activate the BIV promoter effectively, as measured by the expression of the bacterial chloramphenicol acetyltransferase gene, when transfected into bovine cells. Besides activating the BIV promoter, the BIV Tat can also trans activate the HIV promoter effectively. It is possible that BIV Tat and HIV Tat employ similar mechanisms in trans activation of the viral long terminal repeat-directed gene expression.

Original languageEnglish (US)
Pages (from-to)5137-5140
Number of pages4
JournalJournal of virology
Volume66
Issue number8
StatePublished - Jan 1 1992

Fingerprint

Bovine Immunodeficiency Virus
Trans-Activators
immunosuppression
viruses
cattle
Human immunodeficiency virus
Genes
genes
Clone Cells
HIV
promoter regions
clones
Complementary DNA
tat Gene Products
Virus Activation
Chloramphenicol O-Acetyltransferase
chloramphenicol acetyltransferase
Terminal Repeat Sequences
Sequence Homology
terminal repeat sequences

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Identification and characterization of the bovine immunodeficiency-like virus tat gene. / Liu, Z. Q.; Sheridan, D.; Wood, C.

In: Journal of virology, Vol. 66, No. 8, 01.01.1992, p. 5137-5140.

Research output: Contribution to journalComment/debate

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abstract = "A cDNA clone of the bovine immunodeficiency-like virus (BIV) trans- activator gene (tat) was identified and characterized. The tat cDNA clone was generated by splicing, and on the basis of sequence analysis, the Tat protein was found to be encoded entirely by the first exon. It is 103 amino acids in size and shares sequence homology with the human immunodeficiency virus (HIV) Tat. The BIV tat clone can trans activate the BIV promoter effectively, as measured by the expression of the bacterial chloramphenicol acetyltransferase gene, when transfected into bovine cells. Besides activating the BIV promoter, the BIV Tat can also trans activate the HIV promoter effectively. It is possible that BIV Tat and HIV Tat employ similar mechanisms in trans activation of the viral long terminal repeat-directed gene expression.",
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