Identification and characterization of the bovine immunodeficiency-like virus tat gene

Zhen Qian Liu, Deborah Sheridan, Charles Wood

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A cDNA clone of the bovine immunodeficiency-like virus (BIV) irons-activator gene (tat) was identified and characterized. The tat cDNA clone was generated by splicing, and on the basis of sequence analysis, the Tat protein was found to be encoded entirely by the first exon. It is 103 amino acids in size and shares sequence homology with the human immunodeficiency virus (HIV) Tat. The BIV tat clone can trans activate the BIV promoter effectively, as measured by the expression of the bacterial chloramphenicol acetyltransferase gene, when transfected into bovine cells. Besides activating the BFV promoter, the BIV Tat can also trans activate the HIV promoter effectively. It is possible that BIV Tat and HIV Tat employ similar mechanisms in trans activation of the viral long terminal repeat-directed gene expression.

Original languageEnglish (US)
Pages (from-to)5137-5140
Number of pages4
JournalJournal of virology
Volume66
Issue number8
StatePublished - Aug 1 1992

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Bovine Immunodeficiency Virus
tat Genes
immunosuppression
viruses
cattle
Human immunodeficiency virus
Clone Cells
HIV
genes
promoter regions
clones
Complementary DNA
tat Gene Products
Virus Activation
Chloramphenicol O-Acetyltransferase
Terminal Repeat Sequences
chloramphenicol acetyltransferase
Sequence Homology
terminal repeat sequences
Sequence Analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Identification and characterization of the bovine immunodeficiency-like virus tat gene. / Liu, Zhen Qian; Sheridan, Deborah; Wood, Charles.

In: Journal of virology, Vol. 66, No. 8, 01.08.1992, p. 5137-5140.

Research output: Contribution to journalArticle

Liu, Zhen Qian ; Sheridan, Deborah ; Wood, Charles. / Identification and characterization of the bovine immunodeficiency-like virus tat gene. In: Journal of virology. 1992 ; Vol. 66, No. 8. pp. 5137-5140.
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