Identification and characterization of peroxisome proliferator response element in the mouse GLUT2 promoter

Seung Soon Im, Jae Woo Kim, Tae Hyun Kim, Xian Li Song, So Youn Kim, Ha Il Kim, Yong Ho Ahn

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

In the present study, we show that the expression of type 2 glucose transporter isoform (GLUT2) could be regulated by PPAR-γ in the liver. Rosiglitazone, PPAR-γ agonist, activated the GLUT2 mRNA level in the primary cultured hepatocytes and Alexander cells, when these cells were transfected with PPAR-γ/RXR-α. We have localized the peroxisome proliferator response element in the mouse GLUT2 promoter by serial deletion studies and site-directed mutagenesis. Chromatin immunoprecipitation assay using ob/ob mice also showed that PPAR-γ rather than PPAR-α binds to the -197/-184 region of GLUT2 promoter. Taken together, liver GLUT2 may be a direct target of PPAR-γ ligand contributing to glucose transport into liver in a condition when PAPR-γ expression is increased as in type 2 diabetes or in severe obesity.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalExperimental and Molecular Medicine
Volume37
Issue number2
DOIs
Publication statusPublished - Apr 30 2005

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Keywords

  • GLUT2
  • Liver
  • PPAR-γ
  • Promoter
  • Rosiglitazone
  • Type 2 diabetes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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