Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten

R. M. Duar, K. J. Clark, P. B. Patil, C. Hernández, S. Brüning, Thomas E Burkey, N. Madayiputhiya, Stephen L Taylor, Jens Walter

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aim: Identify and characterize bacteria from the proximal gastrointestinal tract of pigs capable of degrading immunogenic gluten peptides. Methods and Results: Bacteria were cultured from the small intestine of pigs fed a 20% gluten diet and from an enrichment media with the 18-mer peptide LQLQPFPQPQLPYPQPQL. Isolates were screened for the production of specialized proteolytic enzymes and the ability to degrade and remove metastable peptides from α-gliadin (16-mer and 33-mer) and ω-gliadin (17-mer), with established roles in the aetiology of coeliac disease. Degradation was determined by ELISA and mass spectrometry (UHPLC-MS/MS in MRM mode), and hydrolysis fragments were characterized by LC-MS/MS. Four strains from the species Lactobacillus ruminis, Lactobacillus johnsonii, Lactobacillus amylovorus and Lactobacillus salivarius showed the highest peptide-degrading activities. Strains displayed different degradation rates and cleavage patterns that resulted in reduction but not complete removal of immunotoxic epitopes. Conclusions: We employed a unique enrichment process to select for bacteria adapted to the conditions of the proximal gastrointestinal tract with the ability to partially detoxify well-characterized peptides involved in coeliac disease. Significance and Impact of the Study: This study provides a basis for the selection of Lactobacillus strains for probiotic applications aimed to reduce epitope-containing gluten peptides before reaching the epithelium of the small intestine of patients with coeliac disease.

Original languageEnglish (US)
Pages (from-to)515-527
Number of pages13
JournalJournal of Applied Microbiology
Volume118
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Glutens
Lactobacillus
Peptides
Celiac Disease
Gliadin
Bacteria
Small Intestine
Gastrointestinal Tract
Epitopes
Swine
Lactobacillus acidophilus
Probiotics
Mass Spectrometry
Hydrolysis
Peptide Hydrolases
Epithelium
Enzyme-Linked Immunosorbent Assay
Diet

Keywords

  • Coeliac
  • Epitopes
  • Gliadin
  • Gluten
  • Lactobacillus
  • Peptidase
  • Probiotics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. / Duar, R. M.; Clark, K. J.; Patil, P. B.; Hernández, C.; Brüning, S.; Burkey, Thomas E; Madayiputhiya, N.; Taylor, Stephen L; Walter, Jens.

In: Journal of Applied Microbiology, Vol. 118, No. 2, 01.02.2015, p. 515-527.

Research output: Contribution to journalArticle

Duar, R. M. ; Clark, K. J. ; Patil, P. B. ; Hernández, C. ; Brüning, S. ; Burkey, Thomas E ; Madayiputhiya, N. ; Taylor, Stephen L ; Walter, Jens. / Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. In: Journal of Applied Microbiology. 2015 ; Vol. 118, No. 2. pp. 515-527.
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