Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten

R. M. Duar, K. J. Clark, P. B. Patil, C. Hernández, S. Brüning, T. E. Burkey, N. Madayiputhiya, S. L. Taylor, Jens Walter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: Identify and characterize bacteria from the proximal gastrointestinal tract of pigs capable of degrading immunogenic gluten peptides. Methods and Results: Bacteria were cultured from the small intestine of pigs fed a 20% gluten diet and from an enrichment media with the 18-mer peptide LQLQPFPQPQLPYPQPQL. Isolates were screened for the production of specialized proteolytic enzymes and the ability to degrade and remove metastable peptides from α-gliadin (16-mer and 33-mer) and ω-gliadin (17-mer), with established roles in the aetiology of coeliac disease. Degradation was determined by ELISA and mass spectrometry (UHPLC-MS/MS in MRM mode), and hydrolysis fragments were characterized by LC-MS/MS. Four strains from the species Lactobacillus ruminis, Lactobacillus johnsonii, Lactobacillus amylovorus and Lactobacillus salivarius showed the highest peptide-degrading activities. Strains displayed different degradation rates and cleavage patterns that resulted in reduction but not complete removal of immunotoxic epitopes. Conclusions: We employed a unique enrichment process to select for bacteria adapted to the conditions of the proximal gastrointestinal tract with the ability to partially detoxify well-characterized peptides involved in coeliac disease. Significance and Impact of the Study: This study provides a basis for the selection of Lactobacillus strains for probiotic applications aimed to reduce epitope-containing gluten peptides before reaching the epithelium of the small intestine of patients with coeliac disease.

Original languageEnglish (US)
Pages (from-to)515-527
Number of pages13
JournalJournal of Applied Microbiology
Volume118
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Glutens
Lactobacillus
Peptides
Celiac Disease
Gliadin
Bacteria
Small Intestine
Gastrointestinal Tract
Epitopes
Swine
Lactobacillus acidophilus
Probiotics
Mass Spectrometry
Hydrolysis
Peptide Hydrolases
Epithelium
Enzyme-Linked Immunosorbent Assay
Diet

Keywords

  • Coeliac
  • Epitopes
  • Gliadin
  • Gluten
  • Lactobacillus
  • Peptidase
  • Probiotics

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. / Duar, R. M.; Clark, K. J.; Patil, P. B.; Hernández, C.; Brüning, S.; Burkey, T. E.; Madayiputhiya, N.; Taylor, S. L.; Walter, Jens.

In: Journal of Applied Microbiology, Vol. 118, No. 2, 01.02.2015, p. 515-527.

Research output: Contribution to journalArticle

Duar, R. M. ; Clark, K. J. ; Patil, P. B. ; Hernández, C. ; Brüning, S. ; Burkey, T. E. ; Madayiputhiya, N. ; Taylor, S. L. ; Walter, Jens. / Identification and characterization of intestinal lactobacilli strains capable of degrading immunotoxic peptides present in gluten. In: Journal of Applied Microbiology. 2015 ; Vol. 118, No. 2. pp. 515-527.
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