Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans

Steven D Harris, Amy F. Hofmann, Hugo W. Tedford, Maurice P. Lee

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In the filamentous fungus Aspergillus nidulans, germination of an asexual conidiospore results in the formation of a hyphal cell. A key feature of spore germination is the switch from isotropic spore expansion to polarized apical growth. Here, temperature-sensitive mutations are used to characterize the roles of five genes (sepA, hypA, podB-podD) in the establishment and maintenance of hyphal polarity. Evidence that suggests that the hypA, podB, and sepA genes are required for multiple aspects of hyphal morphogenesis is presented. Notably, podB and sepA are needed for organization of the cytoskeleton at sites of polarized growth. In contrast, podC and podD encode proteins that appear to be specifically required for the establishment of hyphal polarity during spore germination. The role of sepA and the pod genes in controlling the spatial pattern of polarized morphogenesis in germinating spores is also described. Results obtained from these experiments indicate that the normal pattern of germ-tube emergence is dependent upon the integrity of the actin cytoskeleton.

Original languageEnglish (US)
Pages (from-to)1015-1025
Number of pages11
JournalGenetics
Volume151
Issue number3
StatePublished - Mar 1 1999

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Aspergillus nidulans
Spores
Morphogenesis
Fungi
Germination
Genes
Growth
Cytoskeleton
Actin Cytoskeleton
Maintenance
Mutation
Temperature
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Harris, S. D., Hofmann, A. F., Tedford, H. W., & Lee, M. P. (1999). Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans. Genetics, 151(3), 1015-1025.

Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans. / Harris, Steven D; Hofmann, Amy F.; Tedford, Hugo W.; Lee, Maurice P.

In: Genetics, Vol. 151, No. 3, 01.03.1999, p. 1015-1025.

Research output: Contribution to journalArticle

Harris, SD, Hofmann, AF, Tedford, HW & Lee, MP 1999, 'Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans', Genetics, vol. 151, no. 3, pp. 1015-1025.
Harris, Steven D ; Hofmann, Amy F. ; Tedford, Hugo W. ; Lee, Maurice P. / Identification and characterization of genes required for hyphal morphogenesis in the filamentous fungus Aspergillus nidulans. In: Genetics. 1999 ; Vol. 151, No. 3. pp. 1015-1025.
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