Identification and characterization of a family of toxin-antitoxin systems related to the Enterococcus faecalis plasmid pAD1 par addiction module

Keith E. Weaver, Shirisha G. Reddy, Cassandra L. Brinkman, Smita Patel, Kenneth W Bayles, Jennifer L. Endres

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The par locus of the Enterococcus faecalis plasmid pAD1 is an RNA-regulated addiction module encoding the peptide toxin Fst. Homology searches revealed that Fst belongs to a family of at least nine related peptides encoded on the chromosomes and plasmids of six different Gram-positive bacterial species. Comparison of an alignment of these peptides with the results of a saturation mutagenesis analysis indicated regions of the peptides important for biological function. Examination of the genetic context of the fst genes revealed that all of these peptides are encoded within par-like loci with conserved features similar to pAD1 par. All four Ent. faecalis family members were demonstrated to produce the expected toxin-encoding and regulatory RNA products. The locus from the Ent. faecalis plasmid pAMS1 was demonstrated to function as an addiction module and Fst was shown to be toxic to Staphylococcus aureus, suggesting that a plasmid-encoded module in that species is performing the same function. Thus, the pAD1-encoded par locus appears to be the prototype of a family of related loci found in several Gram-positive species.

Original languageEnglish (US)
Pages (from-to)2930-2940
Number of pages11
JournalMicrobiology
Volume155
Issue number9
DOIs
StatePublished - Sep 14 2009

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Antitoxins
Enterococcus faecalis
Plasmids
Peptides
RNA
Poisons
Mutagenesis
Staphylococcus aureus
Chromosomes
Genes

ASJC Scopus subject areas

  • Microbiology

Cite this

Identification and characterization of a family of toxin-antitoxin systems related to the Enterococcus faecalis plasmid pAD1 par addiction module. / Weaver, Keith E.; Reddy, Shirisha G.; Brinkman, Cassandra L.; Patel, Smita; Bayles, Kenneth W; Endres, Jennifer L.

In: Microbiology, Vol. 155, No. 9, 14.09.2009, p. 2930-2940.

Research output: Contribution to journalArticle

Weaver, Keith E. ; Reddy, Shirisha G. ; Brinkman, Cassandra L. ; Patel, Smita ; Bayles, Kenneth W ; Endres, Jennifer L. / Identification and characterization of a family of toxin-antitoxin systems related to the Enterococcus faecalis plasmid pAD1 par addiction module. In: Microbiology. 2009 ; Vol. 155, No. 9. pp. 2930-2940.
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