Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis

Surendra K. Shukla, Scott E. Mulder, Pankaj Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells. Here we describe an easy and simple way of measuring the hypoxia-mediated tumor glucose uptake in vivo. The method is based on fluorescent imaging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-113
Number of pages7
Volume1742
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Fingerprint

Glucose
Hypoxia-Inducible Factor 1
Neoplasms
Facilitative Glucose Transport Proteins
Pancreatic Neoplasms
Fluorescent Dyes
Transcription Factors
Hypoxia
Oxygen
Genes
Tumor Hypoxia

Keywords

  • 2-DG
  • Cancer metabolism
  • Glucose uptake in mice tumors
  • HIF-1α
  • Hypoxia
  • In vivo glucose uptake

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shukla, S. K., Mulder, S. E., & Singh, P. (2018). Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. In Methods in Molecular Biology (Vol. 1742, pp. 107-113). (Methods in Molecular Biology; Vol. 1742). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7665-2_10

Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. / Shukla, Surendra K.; Mulder, Scott E.; Singh, Pankaj.

Methods in Molecular Biology. Vol. 1742 Humana Press Inc., 2018. p. 107-113 (Methods in Molecular Biology; Vol. 1742).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shukla, SK, Mulder, SE & Singh, P 2018, Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. in Methods in Molecular Biology. vol. 1742, Methods in Molecular Biology, vol. 1742, Humana Press Inc., pp. 107-113. https://doi.org/10.1007/978-1-4939-7665-2_10
Shukla SK, Mulder SE, Singh P. Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. In Methods in Molecular Biology. Vol. 1742. Humana Press Inc. 2018. p. 107-113. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7665-2_10
Shukla, Surendra K. ; Mulder, Scott E. ; Singh, Pankaj. / Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis. Methods in Molecular Biology. Vol. 1742 Humana Press Inc., 2018. pp. 107-113 (Methods in Molecular Biology).
@inbook{ae4cd6229ae648918c98d3f2df0cf9ea,
title = "Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis",
abstract = "Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors{\^A} that{\^A} contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells. Here we describe an easy and simple way of measuring the hypoxia-mediated tumor glucose uptake in vivo. The method is based on fluorescent imaging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.",
keywords = "2-DG, Cancer metabolism, Glucose uptake in mice tumors, HIF-1α, Hypoxia, In vivo glucose uptake",
author = "Shukla, {Surendra K.} and Mulder, {Scott E.} and Pankaj Singh",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7665-2_10",
language = "English (US)",
volume = "1742",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--113",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Hypoxia-mediated in vivo tumor glucose uptake measurement and analysis

AU - Shukla, Surendra K.

AU - Mulder, Scott E.

AU - Singh, Pankaj

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells. Here we describe an easy and simple way of measuring the hypoxia-mediated tumor glucose uptake in vivo. The method is based on fluorescent imaging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.

AB - Most solid tumors are hypoxic in nature due to the limited supply of oxygen to internal tissues. Hypoxia plays an important role in metabolic adaptations of tumors that contribute significantly to cancer pathogenesis. Among the several metabolic alterations induced by hypoxia, hypoxia-mediated increased glucose uptake serves as the hallmark of metabolic reprogramming. Hypoxia-mediated stabilization of hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor leads to altered expression of several glycolytic genes and glucose transporters, which results in increased glucose uptake by tumor cells. Here we describe an easy and simple way of measuring the hypoxia-mediated tumor glucose uptake in vivo. The method is based on fluorescent imaging probe, RediJect 2-DG, which is a nonradioactive fluorescent-tagged glucose molecule. We have discussed orthotopic tumor implantation of HIF-1α knockdown and control pancreatic cancer cells and glucose uptake measurement in vivo by using IVIS imaging system along with reagent preparations.

KW - 2-DG

KW - Cancer metabolism

KW - Glucose uptake in mice tumors

KW - HIF-1α

KW - Hypoxia

KW - In vivo glucose uptake

UR - http://www.scopus.com/inward/record.url?scp=85040860487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85040860487&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7665-2_10

DO - 10.1007/978-1-4939-7665-2_10

M3 - Chapter

VL - 1742

T3 - Methods in Molecular Biology

SP - 107

EP - 113

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -